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Descripción
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| Chemotaxis enables bacteria to move towards an optimal environment in response to chemical signals. Chemoreceptors constitute the sensory core of the chemotaxis system. One of its domains consists on a sensor domain, or ligand-binding domain (LBD), which detects chemical signals. The contribution of motility and chemotaxis to the pathogenicity of the phytopathogenic bacteria Dickeya dadantii 3937 (Dd3937) has been studied previously in our laboratory. The chemoattraction and chemorepellance in response to compounds such as sugars, amino acids and plant hormones, such as jasmonic acid (JA) has been assessed1. Moreover, in order to identify candidate chemoreceptors sensing wound-derived plant compounds, we carried out a bioinformatics search for candidate chemoreceptors in the genomes of Dd39372 and of Pseudomonas syringae pv.tomato DC3000 (PsPto). We have focused on three predicted chemoreceptors to analyze their specificity for the binding of JA, Xylose and ?-Aminobutiric acid (GABA). The first two are present in Dd3937 and the last one in PsPto. The aim of this work is to study the role of these chemoreceptors in the chemotaxis during the infection and especially during the entry to plant apoplast. We have developed a qualitative assay to evaluate chemotaxis to the plant-derived compounds. To confirm the binding we have carried out Isothermal titration calorimetry (ITC) of recombinant purified LBD and finally we have analyzed the impact of mutations in these chemoreceptors during the early phases of infection. Altogether, our results indicate that the function of these chemoreceptors have a primary role during the infection of host plants. | |
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Internacional
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Si |
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Nombre congreso
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Workshop New Frontiers in Plant Biology |
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Tipo de participación
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970 |
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Lugar del congreso
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Centro de Biotecnología y Genómica de Plantas |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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15/06/2016 |
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Fecha fin congreso
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17/06/2016 |
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Desde la página
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8 |
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Hasta la página
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8 |
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Título de las actas
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Analysis of chemotaxis to plant-derived compounds in Dickeya dadantii and Pseudomonas syringae |