Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Multilevel regulation of a protein component of the polyadenylation machinery in Magnaporthe oryzae
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Rbp35 is an RNA-binding protein required for Magnaporthe oryzae?s full virulence. This protein is a novel protein component of the 3? end processing machinery present exclusively in filamentous fungi [1]. Rbp35 has six tripeptides Arg-gly-Gly (RGG) within a region that regulates nucleocytoplasmic transport and protein degradation. It has a C-terminal autoregulatory domain too. Interestingly, an additional post-transcriptional mechanism is present in Rbp35 5? leader region. Two sense and polyadenylated upstream open reading frames (uORF1 and uORF2) are found within a 233 bp intron located in the 5?UTR of the RBP35 gene. uORF1 can modulate the TOR signaling pathway in the presence of Rbp35 in trans, a surprising feature for an uORF. Furthermore, uORF1 is possibly regulating Rbp35 translation [2]. The aim of my investigation is to study Rbp35 regulation, specifically how 5?UTR uORFs can regulate Rbp35 protein levels in the fungal cell. To achieve this several approaches have been undertaken. First, uORF1 function is being analyzed by site-directed mutagenesis of its start codon and changing conserved sequences within the 5? UTR intron (donor site, acceptor site and branch point) where the uORF1 is located. Second, uORF1 is being tagged with HA to see if it can encode a functional peptide. Third, usually uORFs regulate in cis the main downstream coding sequence by non-sense mediated decay machinery. Expression of several constructs of RBP35 in the ?upf1 mutant background is being analysed to see if the presence or absence of the uORF1 and uORF1 can regulate its mRNA levels.
Nombre congreso
Workshop New Frontiers in Plant Biology
Tipo de participación
Lugar del congreso
Centro de Biotecnología y Genómica de Plantas
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Título de las actas
Multilevel regulation of a protein component of the polyadenylation machinery in Magnaporthe oryzae
Esta actividad pertenece a memorias de investigación
  • Autor: Julio Luis Rodriguez Romero (UPM)
  • Autor: Ane Sesma Galarraga (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2023 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)