Observatorio de I+D+i UPM

Descripción
?Rhizobia? are a diverse group of soil ?- and ?-proteobacteria able to establish endosymbiosis with plants belonging to the Fabaceae (legumes) group. In this symbiosis legumes are able to feed from the nitrogen fixed by the rhizobia, which makes them independent from any external fixed nitrogen supply. Common rhizobia belong to the genera Rhizobium, Sinorhizobium, Neorhizobium, Mesorhizobium and Bradyrhizobium. The detection of endophytic and exophytic rhizobia in many of the non-legume plant microbiome projects carried out lately (1,2,3,4) might suggest that rhizobia could be universally present in the plant microbiomes. For symbiotic rhizobia isolation, ?plant traps? have traditionally been used. For the non-symbiotic rhizobia we have developed a strategy consisting of a combination of semi-selective media already described, with the use of specific genus markers developed in our laboratory. This will allow us to characterise the symbiotic and non-symbiotic rhizobia populations in a given soil. This soil will then be used as the source of rhizobia where different plants will be grown in order to determine their effect on these populations and to uncover the biological role of rhizobia with plants. We describe the characterisation of R. leguminosarum, Sinorhizobium sp., Neorhizobium sp., and Bradyrhizobium sp., symbiotic and non-symbiotic populations of an agricultural soil where no legumes have been grown. We find a general paucity of symbiotic rhizobia: R. leguminosarum and Sinorhizobium sp., numbers are low and no Bradyrhizobium sp. strains have been isolated. The observed high abundance of non-symbiotic Neorhizobium sp. strains isolated suggests an important role of these rhizobia in this soil.
Internacional	Si
Nombre congreso	Workshop New Frontiers in Plant Biology
Tipo de participación	970
Lugar del congreso	Centro de Biotecnología y Genómica de Plantas
Revisores	Si
ISBN o ISSN	0000-0000
DOI
Fecha inicio congreso	15/06/2016
Fecha fin congreso	17/06/2016
Desde la página	39
Hasta la página	39
Título de las actas	Characterisation of Symbiotic and Non-symbiotic Rhizobial Diversity in an Agricultural Soil

Esta actividad pertenece a memorias de investigación

• Autor: Amalia Soenens Martínez de Murguia (UPM)

• Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP