Descripción
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In eukaryotes, polyadenylation process defines the end of messenger RNA in a highly regulated manner. Polyadenylation site election influences in RNA translocation, stability and protein translation. The use of different polyadenylation sites has been related to cell differentiation, division, plant growth and response to many stresses. Despite of its great importance, the polyadenylation process and site usage is poorly understood, likely because the majority of the mutants involved in this process are lethal. Here, we have identified a single nucleotide mutation in FIP1, one of the key proteins of the polyadenylation machinery, which leads to alternative polyadenylation (APA) of a large number of genes involved in the regulation of plant development and responses to different abiotic stresses. By bioinformatics means we have found that fip1-1 mutant prefers to use distal polyadenylation sites rather than proximal ones. The fip1-1 mutation alters a large number of biological processes, such as seed dormancy, lateral root formation, leaves growth and flower development among many other processes. In addition, we have found that fip1-1 affects plant responses to salt stress or nitrate starvation. In summary, fip1-1 mutation causes a severe pleiotropic phenotype, likely as consequence of the generation of new proteins isoforms, differential protein translation or RNA stability. In fact, RNAseq analysis shows that fip1-1 mutation affects transcript accumulation of a large number of genes that belongs to many different functional categories. Our results will contribute to understand the role of APA in plant development and also in responses to different abiotic stresses. | |
Internacional
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Si |
Nombre congreso
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Workshop New Frontiers in Plant Biology |
Tipo de participación
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970 |
Lugar del congreso
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Centro de Biotecnología y Genómica de Plantas |
Revisores
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Si |
ISBN o ISSN
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0000-0000 |
DOI
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Fecha inicio congreso
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15/06/2016 |
Fecha fin congreso
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17/06/2016 |
Desde la página
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43 |
Hasta la página
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43 |
Título de las actas
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Alternative polyadenylation regulates plant development and responses to abiotic stresses |