Observatorio de I+D+i UPM

Descripción
An integrated optical biosensor is used for label-free detection and a competitive limit of detection is obtained. The purpose sensor is fabricated by contact lithography technique [1] and a bio-compatible multilayer interferometric transducer is created. The resulting biosurface makes the immobilization of any possible bioreceptor easier and allows a vertical optical characterization of the biosensor. In this case a label-free detection of protein S100A6 related with dry eye disease is performed. To accomplish the S100A6 detection, in first place only 3 µL of protein A is incubated. Through this linker, a desired orientation of the antibody (bioreceptor) can lead to a better recognition. After protein A , 3 µL of 20 µg/mL antiS100A6 solution is incubated. The biofunctionalization process finishes with the incubation of 3% BSA solution as blocking agent to avoid unspecific recognition. Finally, the detection of the S100A6 is performed using different concentrations solutions of 3 µL. As a result, the recognition curve shows that the proposed biosensor is capable to detect the protein in a detection range from ng/mL to µg/mL with very low sample volume.
Internacional	Si
Nombre congreso	II Workshop in Biomaterial an Applications
Tipo de participación	970
Lugar del congreso	Santiago de Compostela
Revisores	No
ISBN o ISSN	000-00-00000-00-0
DOI
Fecha inicio congreso	27/04/2017
Fecha fin congreso	28/04/2017
Desde la página	51
Hasta la página	51
Título de las actas	BioPhotonics Sensing Cells for in-situ dry eye screening II Workshop in Biomaterial an Applications

Esta actividad pertenece a memorias de investigación

• Autor: Beatriz Santamaria Fernandez (UPM)
• Autor: Maria Fe Laguna Heras (UPM)
• Autor: David López-Romero (BioD)
• Autor: Ana Lopez Hernandez (UPM)
• Autor: Francisco Javier Sanza Gutierrez (UPM)
• Autor: Alvaro Lavin Hueros (UPM)
• Autor: Rafael Casquel Del Campo (UPM)
• Autor: Mª Victoria Maigler López (UPM)
• Autor: Rocio Lopez Espinosa (UPM)
• Autor: Miguel Holgado Bolaños (UPM)

• Creador: Grupo de Investigación: Grupo de Óptica Fótonica y Biofotónica (GOFB)
• Centro o Instituto I+D+i: Centro Laser
• Departamento: Física Aplicada e Ingeniería de Materiales
• Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
• Departamento: Tecnología Fotónica y Bioingeniería