Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
BioPhotonics Sensing Cells for in-situ dry eye screening
Año:2017
Áreas de investigación
  • Biosensores
Datos
Descripción
An integrated optical biosensor is used for label-free detection and a competitive limit of detection is obtained. The purpose sensor is fabricated by contact lithography technique [1] and a bio-compatible multilayer interferometric transducer is created. The resulting biosurface makes the immobilization of any possible bioreceptor easier and allows a vertical optical characterization of the biosensor. In this case a label-free detection of protein S100A6 related with dry eye disease is performed. To accomplish the S100A6 detection, in first place only 3 µL of protein A is incubated. Through this linker, a desired orientation of the antibody (bioreceptor) can lead to a better recognition. After protein A , 3 µL of 20 µg/mL antiS100A6 solution is incubated. The biofunctionalization process finishes with the incubation of 3% BSA solution as blocking agent to avoid unspecific recognition. Finally, the detection of the S100A6 is performed using different concentrations solutions of 3 µL. As a result, the recognition curve shows that the proposed biosensor is capable to detect the protein in a detection range from ng/mL to µg/mL with very low sample volume.
Internacional
Si
Nombre congreso
II Workshop in Biomaterial an Applications
Tipo de participación
970
Lugar del congreso
Santiago de Compostela
Revisores
No
ISBN o ISSN
000-00-00000-00-0
DOI
Fecha inicio congreso
27/04/2017
Fecha fin congreso
28/04/2017
Desde la página
51
Hasta la página
51
Título de las actas
BioPhotonics Sensing Cells for in-situ dry eye screening II Workshop in Biomaterial an Applications
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Beatriz Santamaria Fernandez (UPM)
  • Autor: Maria Fe Laguna Heras (UPM)
  • Autor: David López-Romero (BioD)
  • Autor: Ana Lopez Hernandez (UPM)
  • Autor: Francisco Javier Sanza Gutierrez (UPM)
  • Autor: Alvaro Lavin Hueros (UPM)
  • Autor: Rafael Casquel Del Campo (UPM)
  • Autor: Mª Victoria Maigler López (UPM)
  • Autor: Rocio Lopez Espinosa (UPM)
  • Autor: Miguel Holgado Bolaños (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Grupo de Óptica Fótonica y Biofotónica (GOFB)
  • Centro o Instituto I+D+i: Centro Laser
  • Departamento: Física Aplicada e Ingeniería de Materiales
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
  • Departamento: Tecnología Fotónica y Bioingeniería
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)