Artículos en revistas:
Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing
Validation of the Hirst-Type Spore Trap for Simultaneous Monitoring of Prokaryotic and Eukaryotic Biodiversities in Urban Air Samples by Next-Generation Sequencing
Año:2017
Áreas de investigación
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Biología molecular, -
Microbiología,
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Paleobotánica,
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Caracterización de biodiversidad,
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Biotecnología,
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Calidad del medio ambiente,
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Contaminación atmosférica
Datos
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Descripción
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| Pollen, fungi, and bacteria are the main microscopic biological entities present in outdoor air, causing allergy symptoms and disease transmission and having a significant role in atmosphere dynamics. Despite their relevance, a method for monitoring simultaneously these biological particles in metropolitan environments has not yet been developed. Here, we assessed the use of the Hirst-type spore trap to characterize the global airborne biota by high-throughput DNA sequencing, selecting regions of the 16S rRNA gene and internal transcribed spacer for the taxonomic assignment. We showed that aerobiological communities are well represented by this approach. The operational taxonomic units (OTUs) of two traps working synchronically compiled >87% of the total relative abundance for bacterial diversity collected in each sampler, >89% for fungi, and >97% for pollen. We found a good correspondence between traditional characterization by microscopy and genetic identification, obtaining more-accurate taxonomic assignments and detecting a greater diversity using the latter. We also demonstrated that DNA sequencing accurately detects differences in biodiversity between samples. We concluded that high-throughput DNA sequencing applied to aerobiological samples obtained with Hirst spore traps provides reliable results and can be easily implemented for monitoring prokaryotic and eukaryotic entities present in the air of urban areas. | |
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Internacional
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Si |
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JCR del ISI
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Si |
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Título de la revista
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Applied And Environmental Microbiology |
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ISSN
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0099-2240 |
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Factor de impacto JCR
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3,807 |
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Información de impacto
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Volumen
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83 |
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DOI
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10.1128/AEM.00472-17 |
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Número de revista
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13 |
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Desde la página
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e00472-17 |
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Hasta la página
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14 |
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Mes
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ABRIL |
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Ranking
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33/160 Biotechnology & Applied Microbiology |
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Participantes
- Autor: Andrés Nuñez Hernández (UPM)
- Autor: Guillermo Amo de Paz (Facultad de Farmacia (Universidad Complutense de Madrid))
- Autor: Zuzana Ferencova (Facutad de Farmacia (Universidad Complutense de Madrid))
- Autor: Alberto Rastrojo (Centro de Biología Molecular Severo Ochoa (CBMSO-CSIC))
- Autor: Raúl Guantes (Facultad de Ciencias (Universidad Autónoma de Madrid))
- Autor: Ana Maria Garcia Ruiz (UPM)
- Autor: Antonio Alcamí (Centro de Biología Molecular Severo Ochoa (CBMSO-CSIC))
- Autor: A. Montserrat Gutiérrez-Bustillo (Facultad de Farmacia (Universidad Complutense de Madrid))
- Autor: Diego Alejandro Moreno Gomez (UPM)
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