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Abstract
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| Copper is an essential nutrient for symbiotic nitrogen fixation. This element is delivered by the host plant to the nodule, where membrane copper transporter would introduce it into the cell to synthesize cupro-proteins. COPT family members are likely candidates to mediate copper uptake by rhizobiainfected cells. Medicago truncatula genome encodes eight COPT transporters. MtCOPT1 (Medtr4g019870) is the only nodule-specific COPT gene. Promoter:gus fusions and protein immunolocalization studies indicate that MtCOPT1 is located in the plasma membrane of cells in the differentiation, interzone and early fixation zones of the nodule. Loss of MtCOPT1 function results in a copper-mitigated reduction of biomass production when the plant obtains its nitrogen exclusively from symbiotic nitrogen fixation. Mutation of MtCOPT1 results in diminished nitrogenase activity in nodules, likely an indirect effect from the loss of a copper-dependent function, such as cytochrome oxidase activity in copt1-1 bacteroids. These data are consistent with a model in which MtCOPT1 transports copper from the apoplast into nodule cells to provide copper for essential metabolic processes associated with symbiotic nitrogen fixation. | |
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International
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Si |
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Congress
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International Nitrogen Fixation Conference |
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960 |
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Place
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Reviewers
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ISBN/ISSN
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0000000000 |
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Start Date
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03/09/2017 |
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End Date
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07/09/2017 |
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From page
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147 |
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To page
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147 |
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International Nitrogen Fixation Conference |