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Memorias de investigación
Ponencias en congresos:
Exploring the role of MtZifl1, a nodule-specific transporter of the metal chelator nicotianamine
Año:2017
Áreas de investigación
  • Biología molecular, celular y genética,
  • Microbiología
Datos
Descripción
Legumes are one of the largest families in plants, that stands out by their ability to develop root nodules. In these organs, the rhizobial microsymbionts convert the atmospheric nitrogen into ammonia. Nodule formation and functioning is a highly-coordinated process between both partners, starting with a chemical crosstalk which allows rhizobial invasion and nodule organogenesis. The exchange of chemical signals remains throughout the nodulation process, along with the supply of several nutrients from the plant to the bacterial symbiont. Among these nutrients, transition metals are included, since they are essential cofactors of enzymes involved in nodule functioning (1). This notion is supported by several recent findings in our group, using M. truncatula mutants affected in a wide range of genes encoding metal transporters ((2); unpublished data). However, metals are not free in the cells because of their potential toxicity but rather associated with organic ligands such as nicotianamine (NA). NA can form stable complexes with several metals (manganese, iron, cobalt, zinc, nickel and cooper). It has been shown in Arabidopsis thaliana that Zinc-Induced Facilitator 1 (ZIF1) mediates NA distribution (3). In the genome of M. truncatula 8 ZIF-like (ZIFL) genes were identified. Using the available transcriptome database, we found that MtZIfL1 was the only member of this family mainly expressed in the nodule. In addition, the transcript was mostly restricted to the interzone and zone III, were bacteroids accomplish nitrogen fixation. These findings prompted us to unravel the function of MtZIfL1 transporter in nodulation, different approaches are in progress such as characterization of MtZIfL1 mutant, RNAi silencing, and analyses of promoter activity and protein localization.
Internacional
Si
Nombre congreso
International Congress on Nitrogen Fixation
Tipo de participación
970
Lugar del congreso
Revisores
Si
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
03/09/2017
Fecha fin congreso
07/09/2017
Desde la página
164
Hasta la página
164
Título de las actas
International Congress on Nitrogen Fixation
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Juan Imperial Ródenas (UPM)
  • Autor: Jesus Montiel Gonzalez (UPM)
  • Autor: Isidro Abreu Sanchez (UPM)
  • Autor: Manuel Gonzalez Guerrero (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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