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Abstract
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| Zinc (Zn) is an essential element in the structure and catalytic activitiy of a large amount of proteins (1), including many involved in Symbiotic Nitrogen Fixation (SNF) (2). Although much is known about Zn transport and delivery in plants, few studies have focused on how this is achieved in legume nodules. There are three mayor protein families involved in the Zn transport in plants: ZIP, P1b-2ATPases and CDF transporters (3). Characterization of the Zn Medicago truncatula transcriptome showed high expression level of CDF transporter MtMTP2 in nodules. Yeast complementation tests showed that MtMTP2 exports Zn out of the cytosol. To test where MtMTP2 expression is located, ß-glucuronidase activity was determined in M. truncatula plants expresing the gus gene under the MtMTP2 promoter. GUS signal was detected in the differenciation, interzone and fixation zones. Immunolocation studies indicate that MtMTP2 was located in the plasma membrane of non-infected nodule cells and in an intracellular compartment in rhizobia-infected cells. Under symbiotic conditions, the mtp2-1 and mtp2-2 mutants exhibited reduced growth and nitrogenase activity compared to the wild type, but not when mineral nitrogen was provided in nutritive solution. These data indicate an important role of MtMTP2 in the Zn homeostasis in SNF. | |
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International
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Si |
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Congress
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Reunión de la Sociedad Española de Fisiología Vegetal |
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970 |
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Place
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Reviewers
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Si |
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ISBN/ISSN
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0000000000 |
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Start Date
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26/06/2017 |
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End Date
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29/06/2017 |
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From page
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213 |
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To page
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213 |
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Reunión de la Sociedad Española de Fisiología Vegetal |