Memorias de investigación
Research Publications in journals:
Tpc1 is an important Zn(II)2Cys6 transcriptional regulator required for polarized growth and virulence in the rice blast fungus
Year:2017

Research Areas
  • Molecular, cellular and genetic biology

Information
Abstract
The establishment of polarity is a critical process in pathogenic fungi, mediating infection-related morphogenesis and host tissue invasion. Here, we report the identification of TPC1 (Transcription factor for Polarity Control 1), which regulates invasive polarized growth in the rice blast fungus Magnaporthe oryzae. TPC1 encodes a putative transcription factor of the fungal Zn(II)2Cys6 family, exclusive to filamentous fungi. Tpc1-deficient mutants show severe defects in conidiogenesis, infection-associated autophagy, glycogen and lipid metabolism, and plant tissue colonisation. By tracking actin-binding proteins, septin-5 and autophagosome components, we show that Tpc1 regulates cytoskeletal dynamics and infection-associated autophagy during appressorium-mediated plant penetration. We found that Tpc1 interacts with Mst12 and modulates its DNA-binding activity, while Tpc1 nuclear localisation also depends on the MAP kinase Pmk1, consistent with the involvement of Tpc1 in this signalling pathway, which is critical for appressorium development. Importantly, Tpc1 directly regulates NOXD expression, the p22phox subunit of the fungal NADPH oxidase complex via an interaction with Mst12. Tpc1 therefore controls spatial and temporal regulation of cortical F-actin through regulation of the NADPH oxidase complex during appressorium re-polarisation. Consequently, Tpc1 is a core developmental regulator in filamentous fungi, linking the regulated synthesis of reactive oxygen species and the Pmk1 pathway, with polarity control during host invasion.
International
Si
JCR
Si
Title
Plos Pathogens
ISBN
1553-7366
Impact factor JCR
7,003
Impact info
Datos JCR del año 2015
Volume
13
10.1371/journal.ppat.1006516
Journal number
From page
1
To page
31
Month
JULIO
Ranking
Participants
  • Autor: Rita Galhano School of Biosciences, University of Exeter, United Kingdom
  • Autor: Adriana Illana Otero UPM
  • Autor: Lauren S. Ryder School of Biosciences, University of Exeter, United Kingdom
  • Autor: Julio Luis Rodriguez Romero UPM
  • Autor: Marie Demuez . UPM
  • Autor: Muhammad Badaruddin School of Biosciences, University of Exeter, United Kingdom
  • Autor: Ana Lilia Martinez-Rocha School of Biosciences, University of Exeter, United Kingdom
  • Autor: Darren M. Soanes School of Biosciences, University of Exeter, United Kingdom
  • Autor: David J. Studholme School of Biosciences, University of Exeter, United Kingdom
  • Autor: Nicholas J. Talbot School of Biosciences, University of Exeter, United Kingdom
  • Autor: Ane Sesma Galarraga UPM

Research Group, Departaments and Institutes related
  • Creador: Departamento: Biotecnología - Biología Vegetal