Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis.
Año:2018
Áreas de investigación
  • Biología molecular, celular y genética
Datos
Descripción
Body regeneration through formation of new organs is a major question in developmental biology. We investigated de novo root formation using whole leaves of Arabidopsis (Arabidopsis thaliana). Our results show that local cytokinin biosynthesis and auxin biosynthesis in the leaf blade followed by auxin long-distance transport to the petiole leads to proliferation of J0121-marked xylem-associated tissues and others through signaling of INDOLE-3-ACETIC ACID INDUCIBLE28 (IAA28), CRANE (IAA18), WOODEN LEG, and ARABIDOPSIS RESPONSE REGULATORS1 (ARR1), ARR10, and ARR12. Vasculature proliferation also involves the cell cycle regulator KIP-RELATED PROTEIN2 and ABERRANT LATERAL ROOT FORMATION4, resulting in a mass of cells with rooting competence that resembles callus formation. Endogenous callus formation precedes specification of postembryonic root founder cells, from which roots are initiated through the activity of SHORT-ROOT, PLETHORA1 (PLT1), and PLT2. Primordia initiation is blocked in shr plt1 plt2 mutant. Stem cell regulators SCHIZORIZA, JACKDAW, BLUEJAY, and SCARECROW also participate in root initiation and are required to pattern the new organ, as mutants show disorganized and reduced number of layers and tissue initials resulting in reduced rooting. Our work provides an organ regeneration model through de novo root formation, stating key stages and the primary pathways involved.
Internacional
Si
JCR del ISI
Si
Título de la revista
Plant Physiology
ISSN
0032-0889
Factor de impacto JCR
5,9
Información de impacto
Volumen
176(2)
DOI
10.1104/pp.17.00980
Número de revista
Desde la página
1709
Hasta la página
1727
Mes
SIN MES
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Estefano Bustillo Avendaño (UPM)
  • Autor: Sergio Ibañez (Instituto de Bioingeniería, Universidad Miguel Hernández,)
  • Autor: Oscar Sanz
  • Autor: Jessica Aline Sousa Barros (Instituto de Bioingeniería, Universidad Miguel Hernández,)
  • Autor: Inmaculada Gude Rodriguez (UPM)
  • Autor: Juan Periañez Rodriguez (UPM)
  • Autor: José Luis Micol (Instituto de Bioingeniería, Universidad Miguel Hernández,)
  • Autor: Juan Carlos Del Pozo Benito (UPM)
  • Autor: Jose Manuel Peréz Peréz (Instituto de Bioingeniería, Universidad Miguel Hernández,)
  • Autor: Miguel Angel Moreno Risueño (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Departamento: Biotecnología - Biología Vegetal
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)