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RAV1 dose is required for poplar adaptation to low ambient temperature during growth-dormancy transition
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Fine-tuning of environmental signals maximize plant growth and guarantee their survival. To anticipate the winter, poplar use photoperiodic information as a warning system to trigger cold hardiness and dormancy. When photoperiod fall below a critical day-length poplar ceases growth and initiates a cascade of physiological and morphological transformation leading to cold acclimation and bud set. Temporal transcriptome analyses unraveled the molecular framework operating during the photoperiodic-dependent induction of growth cessation, cold acclimation and bud set (Ruttink et al., 2007). However, functional characterization of potential regulatory candidate genes and molecular mechanisms involved are very little investigated. Among these candidates, a related to APETALA2 and Viviparous transcription factor, RAV1, was previously characterized in our laboratory as a regulator of sylleptic branching development in poplar (Moreno-Cortes et al., 2012; Moreno-Cortes et al., 2017). Additionally, we found that RAV1 is induced under SD and cold conditions. Therefore, we investigated its molecular and physiological function during the growth-dormancy transition. In this work, we show that poplar responses to SD and low ambient temperature depend on RAV1 doses. Higher percentage of plants showing leaf injuries were found in RAV1 KD respect to wild type and none in RAV1 OX, suggesting RAV1 mediates early adaptation to low ambient temperature. RNA-seq analyses showed RAV1 doses are correlated with doses of poplar orthologs to ETHYLENE RESPONSE FACTOR TYPE VII transcription factor and plasma membrane RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN-LIKE (RBOH-like) which have been shown to create ROS-dependent tolerance to abiotic stress in Arabidopsis (Yao et al., 2017). Conditional activation of RAV1 showed that ERF and RBOH genes are activated upon detection of continuous high RAV1 levels during several days. Moreover, our time course expression studies showed that low ambient temperature causes high accumulation of RAV1 transcript. Thus, we propose that RAV1-ERF-RBOH module act as a system to monitor low ambient temperature under short days contributing to the early adaptation of plant during growth-dormancy transition in poplar.
Nombre congreso
XIV Reunión de Biología Molecular de Plantas
Tipo de participación
Lugar del congreso
Salamanca (Spain)
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Título de las actas
XIV Reunión de Biología Molecular de Plantas
Esta actividad pertenece a memorias de investigación
  • Autor: Paolo Maria Triozzi . (UPM)
  • Autor: Daniel Conde (University of Florida)
  • Autor: Alicia Moreno-Cortés (Universidad Politecnica de Madrid)
  • Autor: Miguel Angel Torres Lacruz (UPM)
  • Autor: Pablo Gonzalez-Melendi De Leon (UPM)
  • Autor: Jerald D. Noble (University of Florida)
  • Autor: Chris Dervinis (University of Florida)
  • Autor: Matias Kirst (University of Florida)
  • Autor: Isabel Marta Allona Alberich (UPM)
  • Autor: Mariano Manuel Perales (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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