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Memorias de investigación
Ponencias en congresos:
Mitogen-activated protein kinase phosphatase 1 (MKP1) negatively regulates Microbe-Associated Molecular Pattern-triggered immunity in Arabidopsis.
Año:2018
Áreas de investigación
  • Biología molecular, celular y genética
Datos
Descripción
In the search for early signalling components of Microbe-Associated Molecular Pattern (MAMP)-triggered immunity we identified a null allele of the mitogen-activated protein kinase phosphatase 1 gene (MKP1). MKP1 appears as a negative regulator of broad MAMP-triggering immunity responses since mkp1 mutant is more resistant to the necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM), the hemibiotrophic bacterium Pseudomonas syringae pv. tomato DC3000 and the oomycete Hyaloperonospora arbidopsidis. MKP1 regulates ROS production by modulating the NADPH oxidase RBOHD, since mkp1 plants constitutively overexpressing RbohD (35S::RbohD mkp1) display elevated ROS levels upon MAMP treatment. A metabolomic analysis reveals a significant reprograming of the metabolic profile in mkp1, with more than 200 metabolites showing differential accumulation compared to wild-type plants. Antimicrobial compounds of the glucosinolate pathway or camalexin as well as defense-associated metabolites, like saliciylic acid (SA) are among the compounds that mkp1 plants accumulate at high levels. To characterize the elements responsible for this broad enhanced resistance, we crossed mkp1 mutant to lines compromised in production of tryptophan derived metabolites and SA. Patho-tests performed in the combinatory mutants revealed that distinct elements are required for the enhanced resistance to the different pathogens.
Internacional
No
Nombre congreso
XIII Congreso de Biología Molecular de Plantas
Tipo de participación
970
Lugar del congreso
Salamanca
Revisores
Si
ISBN o ISSN
000-00-0000-000-0
DOI
Fecha inicio congreso
04/07/2018
Fecha fin congreso
06/07/2018
Desde la página
224
Hasta la página
224
Título de las actas
Abstracts
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Miguel Angel Torres Lacruz (UPM)
  • Autor: Viviana Pamela Escudero Welsch (UPM)
  • Autor: M Delgado
  • Autor: Sara Sopeña-Torres
  • Autor: Sanjai Swami (UPM)
  • Autor: Jorge Morales (UPM)
  • Autor: Antonio Muñoz Barrios (UPM)
  • Autor: H Mélida
  • Autor: Lucia Jorda Miro (UPM)
  • Autor: Antonio Molina Fernandez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: BIOLOGÍA MOLECULAR Y COMPUTACIONAL
  • Grupo de Investigación: Patología Vegetal
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