Memorias de investigación
Communications at congresses:
Identification of nitrate-dependent Na+ uptake in Arabidopsis roots through transcriptomic profiling

Research Areas
  • Chemistry,
  • Earth sciences,
  • Plants and animal biology and ecology,
  • Agriculture

Despite intensive research for more than 50 years, how Na+ enters the plant is still unknown. Previously we found out that under saline conditions Na + entrance in Arabidopsis takes place largely in a nitrate-dependent manner (Álvarez-Aragón and Rodriguez-Navarro, 2017), suggesting that Na+ may enter the root through a nitrate transporter. A large family of at least 53 genes in Arabidopsis encodes nitrate transporters. Therefore, the identification of which transporters are involved in Na+ uptake may be a hard task. In this study we performed a transcriptomic analysis to identify transporters differentially expressed during Na+ root entry. Using Illumina RNA-Seq transcriptome profiling, we compared the expression of genes encoding putative transporters or channels in hydroponic-grown plants in control conditions (MES without Na+ ) or in two different saline conditions, nitrate independent NaMES (MES with 20 mM Na+ ) or nitrate dependent NaNO3 (20 mM NO3 and 20 mM Na+ ). A total of 57 and 49 differentially expressed genes (DEGs) were identified under Na-MES and NaNO3 treatments compared to control with less than 20% overlap between both gene sets. Indeed, 42 DEGs encoding transporters were identified in a comparison between NaNO3 and Na-MES conditions. We hypothesized that the transporters responsible for nitrate-dependent Na+ entrance will be among this latter group and therefore we have selected some genes for further study. In addition, we also identified candidates for nitrate-independent Na+ uptake among DEGs from Na-MES vs control comparison. We are carrying out a phenotypic study of Na+ accumulation of the respective Arabidopsis defective mutants and a functional characterization in Na+ defective yeast mutants that should allow us to identify the transporters involved in the Na+ root entrance by plant roots
XIV Reunión de Biología Molecular de Plantas
Salamanca, España
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Resumen de abstracts de la Reunión

Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal