Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Identification of nitrate-dependent Na+ uptake in Arabidopsis roots through transcriptomic profiling
Año:2018
Áreas de investigación
  • Química,
  • Ciencias de la tierra,
  • Biología vegetal, animal y ecología,
  • Agricultura
Datos
Descripción
Despite intensive research for more than 50 years, how Na+ enters the plant is still unknown. Previously we found out that under saline conditions Na + entrance in Arabidopsis takes place largely in a nitrate-dependent manner (Álvarez-Aragón and Rodriguez-Navarro, 2017), suggesting that Na+ may enter the root through a nitrate transporter. A large family of at least 53 genes in Arabidopsis encodes nitrate transporters. Therefore, the identification of which transporters are involved in Na+ uptake may be a hard task. In this study we performed a transcriptomic analysis to identify transporters differentially expressed during Na+ root entry. Using Illumina RNA-Seq transcriptome profiling, we compared the expression of genes encoding putative transporters or channels in hydroponic-grown plants in control conditions (MES without Na+ ) or in two different saline conditions, nitrate independent NaMES (MES with 20 mM Na+ ) or nitrate dependent NaNO3 (20 mM NO3 and 20 mM Na+ ). A total of 57 and 49 differentially expressed genes (DEGs) were identified under Na-MES and NaNO3 treatments compared to control with less than 20% overlap between both gene sets. Indeed, 42 DEGs encoding transporters were identified in a comparison between NaNO3 and Na-MES conditions. We hypothesized that the transporters responsible for nitrate-dependent Na+ entrance will be among this latter group and therefore we have selected some genes for further study. In addition, we also identified candidates for nitrate-independent Na+ uptake among DEGs from Na-MES vs control comparison. We are carrying out a phenotypic study of Na+ accumulation of the respective Arabidopsis defective mutants and a functional characterization in Na+ defective yeast mutants that should allow us to identify the transporters involved in the Na+ root entrance by plant roots
Internacional
No
Nombre congreso
XIV Reunión de Biología Molecular de Plantas
Tipo de participación
970
Lugar del congreso
Salamanca, España
Revisores
No
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
04/07/2018
Fecha fin congreso
06/07/2018
Desde la página
180
Hasta la página
180
Título de las actas
Resumen de abstracts de la Reunión
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Rocio Alvarez Aragon (UPM)
  • Autor: Begoña Benito Casado (UPM)
  • Autor: Lorena Blazquez Conchillo (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
S2i 2023 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)