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Memorias de investigación
Ponencias en congresos:
Feeding a bioactive extract from Olea europaea to broiler chickens exerts a positive immunoregulatory effect without major effects on gut microbiota.
Año:2018
Áreas de investigación
  • Biología molecular, celular y genética,
  • Biología vegetal, animal y ecología,
  • Agricultura,
  • Ingenierías
Datos
Descripción
The present study examined the effects of bioactive compounds from a standardized Olea europaea extract (OE) on gut microbiota composition and immune response in broiler chickens. A total of 3 hundred and 6 1-dold broiler chickens (Ross 308) were randomly allocated in floor pens (6 pens / treatment, with 17 birds/pen). From 1 to 21 d of age animals were fed with a common non-medicated starter diet based on wheat and soybean meal, and from 22 to 42 d of age with their 3 respective experimental diets: a basal diet with no additives (C) and the same diet with either 100 ppm of monensin (M) or 1000 ppm of OE (Lucta S.A., Spain). At the end of trial, 2 birds per pen (n = 12) were randomly selected to collect blood for measuring circulating concentration of cytokines and glycoprotein and cecal contents for assessing microbiome profile, which was done via DNA extraction and PCR amplification on the V1-V2 regions of 16S rRNA gen. Additionally, ileal mucosa was sampled to analyze gene expression of ASBT, ChREBP, SREBP1, FABP2, FABP6, Claudin1, TGF-?4, Bu-1, TLR4 and 2?, CD3?, IL-2, IL-6, IL8, and IFN-g markers. Analysis of variance was performed with animal as the experimental unit and diet as fixed effect. A mixed-model was used to determine differences between M and OE in gene expression relative to C and to analyze plasma biomarkers. Mean treatment effects were separated using Tukey test at ? = 0.05. Microbial raw sequencing reads were demultiplexed, quality-filtered and analyzed using QIIME 1.9.1. Feeding OE did not affect gut microbiota abundance and diversity, being the most abundant phylum Clostridiciaceae in C (41.7%) and M (51.1%) treatments, and Lactobacillaceae in OE (65.5%) treatment. Plasma concentration and the expression of IL-8 in ileum were significantly higher (P ? 0.05) in birds fed the C diet compared with OE and M fed birds. Also, the expression of anti-inflammatory TGF-?4 (P < 0.01) and the B-cell marker (Bu-1) (P < 0.001) were upregulated in birds fed the OE and M diets compared with the control. No significant effects were observed for the other variables included in this study. In conclusion, the inclusion of a bioactive extract from Olea europaea in broiler diets appears to exert a positive immunoregulatory effect without affecting gut microbiota.
Internacional
Si
Nombre congreso
Poultry Science Association 107th Annual Meeting
Tipo de participación
970
Lugar del congreso
San Antonio, Texas
Revisores
Si
ISBN o ISSN
0032-5791
DOI
Fecha inicio congreso
23/07/2018
Fecha fin congreso
26/07/2018
Desde la página
179
Hasta la página
179
Título de las actas
Poultry Science
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Javier Herrero Encinas (UPM)
  • Autor: Marta Blanch (Lucta S.A.)
  • Autor: José Pastor (Lucta S.A.)
  • Autor: Alessandro Mereu (Lucta S.A.)
  • Autor: Ignacio Ipharraguerre (Lucta S.A.)
  • Autor: David Menoyo Luque (UPM)
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  • Creador: Departamento: Producción Agraria
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