Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Effects of feeding an Olive pomace extract from Olea europaea to broiler chickens on intestinal mucosa integrity after a short-term fasting period
Áreas de investigación
  • Biología molecular, celular y genética,
  • Alimentación animal,
  • Biología vegetal, animal y ecología,
  • Agricultura
The current need to find alternatives to the use of antibiotics utilization in animal feeds is opening opportunities for feed additives such as phytochemicals in poultry. The present study aims to investigate the effects of supplementing broiler diets with a bioactive pomace-oil extract from Olea europaea (OE) on gut integrity after a challenge of intestinal permeability loss induced by a short-term fasting period. 660 one day old male broiler chicken (Cobb 500) housed in floor pens were randomly assigned to 5 experimental groups (6 pen/treatment, with 22 birds/pen). From a basal diet, four experimental diets were designed, namely a control diet with no additives (CF), supplemented with 100 ppm of monensin (MF) or with 500 (OE500F) and 1500 ppm (OE1500F) of an OE provided by Lucta S. A. (Madrid, Spain). Birds were fed their respective diets ad libitum from 1 to 14 d of age and then were submitted to a 15.5 h short-term fasting period. In addition, a control group was included and fed the control diet without undergo the fasting period (CNF). One hour and half before finishing the fasting period, an oral gavage of lactulose and mannitol sugars was given to two random birds per pen. After the fasting period, selected chickens (n = 12) were slaughtered to collect duodenum to analyze intestinal morphology, ileal mucosa scrapings to analyze the expression of genes involved in gut integrity and immune response and blood to measure sugar permeability marker. Intestinal permeability was significantly (P < 0.001) increased by 15.5 h fasting period as measured by lactulose and mannitol ratio marker. Moreover, duodenal crypt depth significantly (P < 0.001) increased in CF birds compared to CNF. In addition, the expression of Claudin1 was significantly lower (P < 0.05) and inflammatory markers TLR4 and IL-8 were significantly increased (P < 0.05) in CF chickens compared to CNF. Duodenal crypt depth was lower in animals fed the OE500F compared to CF birds (P < 0.05). Furthermore, birds fed the OE500F showed higher serum mannitol and reduced IL-8 expression compared to the CF and OE1500F groups (P = 0.05). In addition, the expression of the B-cell marker (Bu-1) increased linearly with the inclusion of OE in diets. The 15.5 h fasting period increased intestinal permeability triggering an inflammatory response mediated by TLR4. The supplementation with 500 ppm of OE ameliorated some of the negative effects of increased intestinal permeability after a short-term fasting period.
Nombre congreso
6th International Conference on Poultry Intestinal Health
Tipo de participación
Lugar del congreso
Roma, Italia
Fecha inicio congreso
Fecha fin congreso
Desde la página
Hasta la página
Título de las actas
Effects of feeding an Olive pomace extract from Olea europaea to broiler chickens on intestinal mucosa integrity after a short-term fasting period
Esta actividad pertenece a memorias de investigación
  • Autor: Javier Herrero Encinas (UPM)
  • Autor: Marta Blanch (Lucta S.A.)
  • Autor: José Pastor (Lucta S.A.)
  • Autor: David Menoyo Luque (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Producción Animal
  • Departamento: Producción Agraria
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)