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Descripción
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| Nitrogen is an essential macronutrient whose availability in soil is crucial for plant growth and crop productivity. Nitrate is the main source of inorganic nitrogen for land plants. However, it is usually limiting in the majority of the ecosystems, due to a variety of biotic and abiotic factors like microbial consumption, water leaching and soil erosion. Besides a macronutrient, nitrate is also a signal molecule that regulates the expression of a variety of genes that modulate many aspects of metabolism, growth and development. Here we identify the roles of Arabidopsis Transcription factors Cycling DOF Factor 1 and 3 (CDF1 and CDF3) in nitrate responses. Loss-of-function null mutant cdf3-1 and cdf1-R exhibit nitrate-dependent root phenotypes, whereas CDF1 and CDF3 overexpression lines showed increased biomass and altered root architecture. Transcriptomic analyses of overexpressor lines indicated that both factors regulate the expression an important set of carbon and nitrogen regulated genes. Consistently, metabolite profiling disclosed that the total amount of key nitrogen metabolites including glutamate and glutamine were higher in CDF1/3-overexpressing plants in N-sufficient and limiting conditions. By contrast, cdf3-1 and cdf1-R mutants exhibited lower amounts of those metabolites. In tomato, the overexpression of CDF3 gene modified amino acid and sugar content, improving biomass and yield. These results identify CDF1 and CDF3 as important regulatory factors of the nitrate response in Arabidopsis, suggesting that they are potential targets for improving nutrient use efficiency in crops. | |
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Internacional
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Si |
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Nombre congreso
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XI REUNION NACIONAL METABOLISMO DEL NITROGENO |
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Tipo de participación
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970 |
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Lugar del congreso
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Segovia, España |
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Revisores
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Si |
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ISBN o ISSN
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0000-0000 |
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DOI
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Fecha inicio congreso
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16/05/2018 |
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Fecha fin congreso
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18/05/2018 |
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Desde la página
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0 |
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Hasta la página
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0 |
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Título de las actas
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XI REUNION NACIONAL METABOLISMO DEL NITROGENO |