Memorias de investigación
Artículos en revistas:
Inclusion of a Furin Cleavage Site Enhances Antitumor Efficacy against Colorectal Cancer Cells of Ribotoxin ?-Sarcin- or RNase T1-Based Immunotoxins
Año:2019
Áreas de investigación
  • Biomedicina,
  • Biología molecular, celular y genética
Datos
Descripción
Immunotoxins are chimeric molecules that combine the specificity of an antibody to recognize and bind tumor antigens with the potency of the enzymatic activity of a toxin, thus, promoting the death of target cells. Among them, RNases-based immunotoxins have arisen as promising antitumor therapeutic agents. In this work, we describe the production and purification of two new immunoconjugates, based on RNase T1 and the fungal ribotoxin -sarcin, with optimized properties for tumor treatment due to the inclusion of a furin cleavage site. Circular dichroism spectroscopy, ribonucleolytic activity studies, flow cytometry, fluorescence microscopy, and cell viability assays were carried out for structural and in vitro functional characterization. Our results confirm the enhanced antitumor e?ciency showed by these furin-immunotoxin variants as a result of an improved release of their toxic domain to the cytosol, favoring the accessibility of both ribonucleases to their substrates. Overall, these results represent a step forward in the design of immunotoxins with optimized properties for potential therapeutic application in vivo.
Internacional
Si
JCR del ISI
Si
Título de la revista
Toxins
ISSN
2072-6651
Factor de impacto JCR
3,531
Información de impacto
Volumen
DOI
10.3390/toxins11100593
Número de revista
Desde la página
0
Hasta la página
20
Mes
OCTUBRE
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Javier Ruiz de la Herrán Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid
  • Autor: Jaime Maria Tome Amat UPM
  • Autor: Rodrigo Lazaro-Gorines Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid
  • Autor: José G. Gavilanes Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid
  • Autor: Javier Lacadena Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias Químicas, Universidad Complutense de Madrid
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biotecnología Vegetal
  • Departamento: Biotecnología - Biología Vegetal