|
Descripción
|
|
|---|---|
| The acquisition of new hosts conditions virus epidemiology and emergence, hence the interest in understanding the mechanisms behind host-range expansions. Experimental evolution studies have identified antagonistic pleiotropy and epistasis as genetic mechanisms that limit host-range expansions, but studies from virus field populations are few. We compared the performance of sympatric isolates of tobacco mild green mosaic virus (TMGMV) from its reservoir host, Nicotiana glauca and its new host, pepper, showing that acquisition of a new host was not followed by adaptation to it, but was associated with a fitness loss in the original host. Analysis of coat protein ORF and 3?UTR mutations determining host-specific virus multiplication identified antagonistic pleiotropy, epistasis and host-specific epistasis as mechanisms generating across-host fitness trade-offs that may prevent adaptation to pepper and cause a loss of fitness in N. glauca. Assays in greenhouse conditions showed that a polymorphism in the 3?UTR was associated with fitness in pepper, but not in N. glauca. However, analyses of field isolates from N. glauca under controlled conditions showed a temperature-dependent effect of 3?UTR alleles on the virus fitness in this host. Analyses of the TMGMV population infecting N. glauca in the field in winter, spring or summer further indicated that the 3? UTR polymorphism is maintained by host- and abiotic environment-related selection. Results show that a full understanding of virus evolution requires joint consideration of host range and host environment, an underexplored and promising topic of research. | |
|
Internacional
|
Si |
|
Nombre congreso
|
10th WORKSHOP IN VIRUS EVOLUTION |
|
Tipo de participación
|
960 |
|
Lugar del congreso
|
State College, Pennsylvania (USA) |
|
Revisores
|
Si |
|
ISBN o ISSN
|
000-00-0000-000-0 |
|
DOI
|
|
|
Fecha inicio congreso
|
07/03/2019 |
|
Fecha fin congreso
|
10/03/2019 |
|
Desde la página
|
6 |
|
Hasta la página
|
6 |
|
Título de las actas
|
Abstracts |