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Ponencias en congresos:
Identification of glutamate residues relevant for efficient maturation of Rhizobium leguminosarum [NiFe] hydrogenase
Año:2019
Áreas de investigación
  • Ciencias naturales y ciencias de la salud
Datos
Descripción
A critical step in the maturation of Rhizobium leguminosarum NiFe hydrogenase involves the Ni-dependent proteolytic processing of large subunit (LSU) precursor (pre-HupL) by a specific endoprotease (HupD). A structural model for pre-HupL has been built through computational modelling and Molecular Dynamics (MD). In this model, the C-terminal tail of pre-HupL behaves as an Intrinsically Disordered Region (IDR), a type of structure known to facilitate the interaction with other proteins such as proteases. MD simulations show that a highly-stable form of pre-HupL contains a nickel atom linked to two glutamate residues located at the Terminal region and the C-terminal disordered segment of HupL (E27 and E589). Phenotypic analysis of mutants affected in each of these residues showed that both are relevant for Ni-dependent processing of pre-HupL. A stronger phenotype was associated to E27Q mutation, in line with previous work indicating that an equivalent residue would be involved in the translocation of protons in Desulfovibrio hydrogenase. Our results, along with structural modelling studies of the HupL-HupD interaction, suggest that both E27 and E589 could participate in a transient binding site for the initial incorporation of nickel into the enzyme, thus allowing efficient interaction of pre-HupL with the endoprotease. Recent studies showed that Thermococcus kodakarensis HypA interacts with LSU C- and N-terminal domains for the incorporation of nickel.
Internacional
Si
Nombre congreso
Internacional Conference on Hydrogenases
Tipo de participación
960
Lugar del congreso
Lisboa
Revisores
Si
ISBN o ISSN
0000000000
DOI
Fecha inicio congreso
31/03/2019
Fecha fin congreso
04/04/2019
Desde la página
24
Hasta la página
25
Título de las actas
ICH2019 Book of Abstracts
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Marta Albareda Contreras (UPM)
  • Autor: Luis Fernandez Pacios (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Abordajes Multidisciplinares en la Interfaz Planta-Microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología - Biología Vegetal
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