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Descripción
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| The aim of this work was to study the effect of dietary glutamine (gln) level and source on rabbit mortality rates induced by Epizootic Rabbit Enteropathy (ERE) and the detection of C. perfringens from intestinal isolates. The effect of this amino acid on mucosal barrier mechanisms was also assessed by studying the relative PPARã mRNA abundance in ileum and jejunum. Three diets were experimentally tested, a basal diet (C) containing 177 g of CP/kg DM, and 28.8 g of gln/kg DM, and two diets supplemented at 0.5% and 1% above the basal levels (C-G0.5 and C-G1). In addition, to study the effects of including 1% gln as synthetic vs. a natural source, a fourth diet (W) was included in the experiment containing a wheat protein concentrate (VITEN cws). One hundred and ninety-six individually lodged animals (49 animals/treatment) and two hundred and twelve collectively lodged animals (53 cages/treatment) weighing 476±93 g and 498±100 g respectively were blocked by litter and randomly assigned to the experimental diets. Animals were fed the experimental diets for 18 d (28 to 46 d of age). During the second period (46 to 60 d of age) all rabbits were fed the basal diet (C). For the microbiological caecal study 60 rabbits with 44 d of age (15 per treatment) weighing 565±96 g were selected. For the RNA isolation and real-time RT-PCR 7 rabbits per treatment (C, C-G0.5 and CG1) from the microbiological analysis were selected. The supplementation with 0.5 L-gln resulted in a reduction of 1.1 log unit of C. perfringens CFU/g (P=0.05) compared to the basal diet. Also a tendency for a quadratic effect of dietary gln level (P=0.09) was observed. Higher relative abundance of PPARã mRNA was observed in ileum than in jejunum, however, no dietary effects were reported either in ileum or in jejunum. No differences were observed between sick and healthy animals. Our results suggest glutamine plays an important role in the intestinal health without effects on growth performances. However, gln level had no effect on PPARã mRNA abundance partly explained by the lack of a clear inflammatory process in sick animals. Optimal results were obtained with 0.5% of synthetic gln supplementation. No differences between the two sources of gln were detected for any of the traits studied. Therefore, synthetic gln sources might be substituted by natural ones, as wheat protein concentrate, decreasing feeding costs. | |
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Internacional
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Si |
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Nombre congreso
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9th World Rabbit Congress |
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Tipo de participación
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960 |
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Lugar del congreso
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Verona (Italia) |
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Revisores
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Si |
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ISBN o ISSN
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978-88-902814-6-4 |
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DOI
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Fecha inicio congreso
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10/06/2008 |
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Fecha fin congreso
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13/06/2008 |
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Desde la página
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529 |
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Hasta la página
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533 |
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Título de las actas
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Proceedings of the 9th World Rabbit Congress |