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Descripción
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| Label-free integrated optical biosensors have been demonstrated to be very sensitive biosensors presenting advantages as high sensitivity, small size, and high scale integration. Recently, it has been reported an integrated photonic sensor based on a slotwaveguide resonator (Figure 1a). Theorethical calculations show that the most sensitive area of the device is the inside of the slot, being able to detect mass changes of 0.8 pg/mm2, due to the variation in the refraction index the recognition antigen-antibody. Thus, an important challenge is to develop chemical methods that let the immobilisation of bioreceptors in a robust and oriented manner selectively on the nitride guides (figure 1a), and keeping their bioavailability. Our studies employing planar test structures demonstrated that the reaction between the amine groups on the silicon nitride surface and a well known cross-linker, glutaraldehyde, provided the selective immobilisation of the bioreceptors against silicon oxyde. In this work, we employed the optimized immobilisation strategy to demonstrate the detection of label-free molecular binding reactions on the surface of a slot-waveguide ring resonator. Bovine serum albumin (BSA) protein and anti-BSA are used to study the biosensor performance. Figure 1b and 1c illustrates the resonante wavelength shift as a function of anti-BSA and BSA concentration. The estimated limits of detection of the device for anti-BSA and BSA molecules were 28 and 16 pg/mm2, limited by wavelength resolution. These values favorable compare with those of state-of-the-art integrated photonic biosensors based on conventional waveguides. | |
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Internacional
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Si |
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Nombre congreso
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II Workshop of Nanoscience and Analytical Nanotechnology |
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Tipo de participación
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960 |
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Lugar del congreso
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Tarragona |
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Revisores
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Si |
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ISBN o ISSN
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0000000000 |
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DOI
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Fecha inicio congreso
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25/09/2008 |
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Fecha fin congreso
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26/09/2008 |
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Desde la página
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1 |
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Hasta la página
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1 |
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Título de las actas
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ABSTRACTS |