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Memorias de investigación
Artículos en revistas:
Host-dependent expression of Rhizobium leguminosarum bv. viciae hydrogenase is controlled at transcriptional and post-transcriptional levels in legume nodules.
Año:2008
Áreas de investigación
  • Microbiología
Datos
Descripción
The legume host affects the expression of Rhizobium leguminosarum hydrogenase activity in root nodules. High levels of symbiotic hydrogenase activity were detected in R. leguminosarum bacteroids from different hosts, with the exception of lentil (Lens culinaris). Transcription analysis showed that the NifA-regulated R. leguminosarum hydrogenase structural gene promoter (P1) is poorly induced in lentil root nodules. Replacement of the P1 promoter by the FnrN-dependent promoter of the fixN gene restored transcription of hup genes in lentil bacteroids, but not hydrogenase activity. In the PfixN-hupSL strain, additional copies of the hup gene cluster and nickel supplementation to lentil plants increased bacteroid hydrogenase activity. However, the level of activity in lentil still was significantly lower than in pea bacteroids, indicating that an additional factor is impairing hydrogenase expression inside lentil nodules. Immunological analysis revealed that lentil bacteroids contain reduced levels of both hydrogenase structural subunit HupL and nickel-binding protein HypB. Altogether, results indicate that hydrogenase expression is affected by the legume host at the level of both transcription of hydrogenase structural genes and biosynthesis or stability of nickelrelated proteins HypB and HupL, and suggest the existence of a plant-dependent mechanism that affects hydrogenase activity during the symbiosis by limiting nickel availability to the bacteroid.
Internacional
Si
JCR del ISI
Si
Título de la revista
MOLECULAR PLANT-MICROBE INTERACTIONS
ISSN
0894-0282
Factor de impacto JCR
4,275
Información de impacto
Volumen
21
DOI
10.1094/MPMI -21-5-0597
Número de revista
5
Desde la página
597
Hasta la página
604
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Participante: Annita Toffanin (Universidad de Pisa)
  • Autor: Maria Belen Brito Lopez (UPM)
  • Autor: Tomas-Andres Ruiz Argueso (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Juan Imperial Ródenas (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Departamento: Biotecnología
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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