Memorias de investigación
Research Publications in journals:
Adaptation of aphid stylectomy for analyses of proteins and mRNAs in barley phloem sap
Year:2008

Research Areas
  • Molecular biology

Information
Abstract
Sieve tubes are transport conduits not only for photoassimilates but also for macromolecules and other compounds that are involved in sieve tube maintenance and systemic signalling. In order to gain sufficient amounts of pure phloem exudates from barley plants for analyses of the protein and mRNA composition, a previously described stylectomy set-up was optimized. Aphids were placed in sealed cages, which, immediately after microcauterization of the stylets, were flooded with water-saturated silicon oil. The exuding phloem sap was collected with a capillary connected to a pump. Using up to 30 plants and 600 aphids (Rhopalosiphum padi) in parallel, an average of 10 ml of phloem sap could be obtained within 6 h of sampling. In first analyses of the macromolecular content, eight so far unknown phloem mRNAs were identified by cDNA-amplified fragment length polymorphism. Transcripts in barley phloem exudates are related to metabolism, signalling, and pathogen defence, for example coding for a protein kinase and a pathogen- and insect-responsive WIR1A (wheatinduced resistance 1A)-like protein. Further, onedimensional gel electrophoresis and subsequent partial sequencing by mass spectrometry led to the identification of seven major proteins with putative functions in stress responses and transport of mRNAs, proteins, and sugars. Two of the discovered proteins probably represent isoforms of a new phloemmobile sucrose transporter. Notably, two-dimensional electrophoresis confirmed that there are >250 phloem proteins awaiting identification in future studies
International
Si
JCR
Si
Title
JOURNAL OF EXPERIMENTAL BOTANY
ISBN
0022-0957
Impact factor JCR
3,917
Impact info
Volume
59
Journal number
0
From page
3297
To page
3306
Month
ENERO
Ranking
Participants
  • Participante: A.J.E van Bel
  • Participante: S Deshmukh
  • Participante: Knauer T
  • Autor: Julia Kehr . UPM
  • Participante: F Gaupels,
  • Participante: F Waller
  • Participante: K.-H Kogel,

Research Group, Departaments and Institutes related
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP