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Memorias de investigación
Artículos en revistas:
The NADPH-oxidase AtrbohB plays a role in Arabidopsis seed after-ripening
Año:2009
Áreas de investigación
  • Biología molecular,
  • Producción vegetal
Datos
Descripción
Seeds can enter a state of dormancy, in which they do not germinate under optimal environmental conditions. Dormancy can be broken during seed after-ripening in the low-hydrated state. By screening enhancer trap lines of Arabidopsis, we identified a role for the NADPH-oxidase AtrbohB in after-ripening. Semiquantitative PCR was used to investigate AtrbohB transcripts in seeds. These methods were complemented with a pharmacological approach using the inhibitor diphenylene iodonium chloride (DPI) and biomechanical measurements in the Brassicaceae seed model system cress (Lepidium sativum) as well as protein carbonylation assays. atrbohB mutants fail to after-ripen and show reduced protein oxidation. AtrbohB pre-mRNA is alternatively spliced in seeds in a hormonally and developmentally regulated manner. AtrbohB is a major producer of superoxide in germinating Arabidopsis seeds, and inhibition of superoxide production by diphenylene iodonium (DPI) leads to a delay in Arabidopsis and cress seed germination and cress endosperm weakening. Reactive oxygen species produced by AtrbohB during after-ripening could act via abscisic acid (ABA) signalling or post-translational protein modifications. Alternative splicing could be a general mechanism in after-ripening: by altered processing of stored pre-mRNAs seeds could react quickly to environmental changes
Internacional
Si
JCR del ISI
Si
Título de la revista
NEW PHYTOLOGIST
ISSN
0028-646X
Factor de impacto JCR
5,178
Información de impacto
Volumen
184
DOI
10.1111/j.1469-8137.2009.03005.x
Número de revista
0
Desde la página
885
Hasta la página
897
Mes
ENERO
Ranking
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Participantes
  • Participante: Ada Linkies (University of Freiburg)
  • Participante: Gerhard Leubner-Metzger (University of Freiburg)
  • Autor: Miguel Angel Torres Lacruz (UPM)
  • Participante: Kerstin Müller (University of Freiburg)
  • Participante: Anna Catharina Carstens (University of Freiburg)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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