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Abstract
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| Embryogenic cell suspensions of two elite grapevine cultivars (Albariño and tempranillo) were successfully cryopreserved by encapsulation-dehydration. The method implied the encapsulation of alginate beads, subsequent culture in liquid medium with 1 M sucrose for 4 days, and desiccation for 2-4 h in the flow of a laminar-flow bench, before immersion in liquid nitrogen. With this simple method, up to 50% of cell viability in the cryopreserved beads was measured which corresponded to vigorous growth of 100% of beads after culture in semi-solid medium. Cryopreserved cells were used to iniciate cell suspensions and obtain somatic embryos. | |
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International
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Si |
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JCR
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Si |
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Title
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VITIS |
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ISBN
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0042-7500 |
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Impact factor JCR
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0,795 |
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Impact info
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Volume
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48 |
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Journal number
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3 |
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From page
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131 |
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To page
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136 |
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Month
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ENERO |
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Ranking
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