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Memorias de investigación
Artículos en revistas:
Molecular characterization and analysis of somaclonal variation in chrysanthemum cultivars using RAPD markers
Año:2009
Áreas de investigación
  • Producción vegetal
Datos
Descripción
Molecular characterization using RAPD analysis was carried out in eight cut flowers and two pot plant cultivars of chrysanthemum. Three of them (`Refocus¿, `Red Reagan¿, and `Sheena Select¿) were established in vitro and the occurrence of somaclonal variation was studied using the same molecular technique. Two induction media (MS + 0.1 mg/l NAA + 0.1 mg/l BA, and MS + 2.0 mg/l IAA + 0.5 mg/l Kinetin), and two proliferation media (MS + 0.1 mg/l NAA + 0.2 mg/l BA, and MS + 4.0 mg/l IAA + 2.0 mg/l Kinetin) were employed in order to evaluate the effect of the medium composition in the shoots¿ stability. Likewise, the effect of the culture age was considered in assessing genetic stability. Monthly subcultures were carried out, identifying the origin and history of the shoots, throughout a nine-month proliferation period followed by acclimatization. Molecular markers were obtained in every subculture cycle and from the acclimatized plants. Only one shoot from the 7th subculture of the cultivar `Refocus¿ showed a different band pattern. The use of RAPD for chrysanthemum cultivar characterization and somaclonal variation detection is discussed.
Internacional
Si
JCR del ISI
Si
Título de la revista
SCIENTIA HORTICULTURAE
ISSN
0304-4238
Factor de impacto JCR
0,859
Información de impacto
Volumen
122
DOI
Número de revista
2
Desde la página
238
Hasta la página
243
Mes
ENERO
Ranking
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Participantes
  • Autor: Hilda Soledad Miñano Toribio (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Maria Carmen Martin Fernandez (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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