Memorias de investigación
Artículos en revistas:
Role of ENA ATPase in Na+ efflux at high pH in bryophytes
Año:2009

Áreas de investigación
  • Biología celular

Datos
Descripción
Potassium or Na+ efflux ATPases, ENA ATPases, are present in all fungi and play a central role in Na+ efflux and Na? tolerance. Flowering plants lack ENA ATPases but two ENA ATPases have been identified in the moss Physcomitrella patens, PpENA1 and PpENA2. PpENA1 mediates Na+ efflux in Saccharomyces cerevisiae. To propose a general function of ENA ATPases in bryophytes it was necessary to demonstrate that these ATPases mediate Na+ efflux in planta and that they exist in more bryophytes than P. patens. For these demonstrations (1) we cloned a third ATPase from P. patens, PpENA3, and studied the expression pattern of the three PpENA genes; (2) we constructed and studied the single and double Dppena1 and Dppena2 mutants; and (3) we cloned two ENA ATPases from the liverwort Marchantia polymorpha, MpENA1 and MpENA2, and expressed them in S. cerevisiae. The results from the first two approaches revealed that the expression of ENA ATPases was greatly enhanced at high pH and that Na+ efflux at high pH depended on PpENA1. The ENA1 ATPase of M. polymorpha suppressed the defective growth of a S. cerevisiae mutant at high K+ or Na+ concentrations, especially at high K+.
Internacional
Si
JCR del ISI
No
Título de la revista
Plant molecular biology
ISSN
0167-4412
Factor de impacto JCR
0
Información de impacto
Volumen
71
DOI
Número de revista
0
Desde la página
599
Hasta la página
608
Mes
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Participantes

Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Homeostasis iónica y ciclo celular
  • Departamento: Biotecnología