Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Bimolecular Complementation assays to study protein-protein interactions in plants
Año:2009
Áreas de investigación
  • Biología molecular
Datos
Descripción
A wide variety of experimental approaches have been developed to study protein-protein interactions in plants, however most of them require disruption of cells or indirect assays of protein detection. The development of sensitive and versatile methods able to detect these interactions in vivo and in homologous tissue and species, is essential to understand protein functions. The Bimolecular Complementation (BiC) either using fluorescent (BiFC) or luminescent (BiLC) compounds provides a direct approach of visualization of molecular interaction in real time, living cells at the subcellular level and under real physiological conditions. BiFC and BiLC procedures are based on the complementation between two fragments of a fluorescent/luminescent protein that can associate when they are brought together by the interaction between proteins fused to each of the fragments. Recent improvements of this technique, the multicolour BiFC approach (mBiFC), have been developed for the simultaneous visualization of multiple protein interactions in the same cell. The combination of fragments of different types of fluorescent proteins makes possible the simultaneous detection of protein complexes in planta. The great potential of this technology to determine biological interactions will be presented.
Internacional
No
Nombre congreso
XIII Congress of the Spanish Society for Cell Biology
Tipo de participación
960
Lugar del congreso
Murcia
Revisores
No
ISBN o ISSN
9788474011975
DOI
Fecha inicio congreso
16/12/2009
Fecha fin congreso
18/12/2009
Desde la página
48
Hasta la página
48
Título de las actas
XIII Congress of SEBC
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: M. Isabel Diaz Rodriguez (UPM)
  • Autor: Ines Cambra Marin (UPM)
  • Autor: Laura Carrillo Gil (UPM)
  • Autor: Manuel Martinez Muñoz (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.
  • Departamento: Biotecnología
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