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Memorias de investigación
Artículos en revistas:
Role of motility and chemotaxis in the pathogenesis of Dickeya dadantii 3937 (ex Erwinia chrysanthemi 3937)
Año:2009
Áreas de investigación
  • Microbiología
Datos
Descripción
Dickeya dadantii 3937 (ex Erwinia chrysanthemi), a member of the Enterobacteriaceae, causes soft rot in many economically important crops. A successful pathogen has to reach the interior of the plant in order to cause disease. To study the role of motility and chemotaxis in the pathogenicity of D. dadantii 3937, genes involved in the chemotactic signal transduction system (cheW, cheB, cheY and cheZ) and in the structure of the flagellar motor (motA) were mutagenized. All the mutant strains grew like the wild-type in culture media, and the production and secretion of pectolytic enzymes was not affected. As expected, the swimming ability of the mutant strains was reduced with respect to the wild-type: motA (94 %), cheY (80 %), cheW (74 %), cheB (54 %) and cheZ (48 %). The virulence of the mutant strains was analysed in chicory, Saintpaulia and potato. The mutant strains were also tested for their capability to enter into Arabidopsis leaves. All the mutants showed a significant decrease of virulence in certain hosts; however, the degree of virulence reduction varied depending on the virulence assay. The ability to penetrate Arabidopsis leaves was impaired in all the mutants, whereas the capacity to colonize potato tubers after artificial inoculation was affected in only two mutant strains. In general, the virulence of the mutants could be ranked as motA,cheY,cheB5cheW,cheZ, which correlated with the degree to which swimming was affected. These results clearly indicate that motility plays an important role in the pathogenicity of this bacterium.
Internacional
Si
JCR del ISI
Si
Título de la revista
MICROBIOLOGY-SGM
ISSN
1350-0872
Factor de impacto JCR
2,841
Información de impacto
Volumen
155
DOI
DOI 10.1099/mic.0.022244-0
Número de revista
0
Desde la página
434
Hasta la página
442
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Maria Antunez Lamas (UPM)
  • Autor: Emilia Antonia Lopez Solanilla (UPM)
  • Autor: Pablo Rodriguez Palenzuela (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Interacciones Moleculares Planta-Patógeno
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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