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Descripción
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| The genetic analysis of shoots regenerated from cryopreserved apices of chrysanthemum (Dendranthema grandiflora), using the encapsulation-dehydration method, showed important differences compared with the control plants. Two different genetic markers (RAPDs and AFLPs) were used to analyze the whole process from the first step of the protocol (sucrose culture) to the recovery of the cryopreserved material. Analyses were carried out through the whole process. Data obtained with RAPDs markers showed always the same mutation in all the analyzed samples, from the high sucrose concentration treatment onwards. On the other hand, differences revealed with the AFLPs markers seemed quite untargeted through the whole-genome level, although the number of differences increased through the process, being the cryopreservd samples those with higher amount of differences within each line. These results suggest that the genetic instability detected with these analyses could be correlated to the degree of stress suffered by the samples during the cryopreservation protocol. | |
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Internacional
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Si |
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Nombre congreso
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Working Group 1 meeting of COST Action 871 |
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Tipo de participación
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960 |
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Lugar del congreso
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Royal Botanic Gardens in Kew, Wakehurst Place, United Kingdom |
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Revisores
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Si |
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ISBN o ISSN
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0143-2044 |
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DOI
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Fecha inicio congreso
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17/02/2009 |
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Fecha fin congreso
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18/02/2009 |
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Desde la página
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396 |
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Hasta la página
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397 |
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Título de las actas
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Cryoletters, Volume: 30 Issue: 5 |