Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Which role plays osmotic stress on (epi-genetic) instability?
Año:2009
Áreas de investigación
  • Producción vegetal
Datos
Descripción
The genetic analysis of shoots regenerated from cryopreserved apices of chrysanthemum (Dendranthema grandiflora), using the encapsulation-dehydration method, showed important differences compared with the control plants. Two different genetic markers (RAPDs and AFLPs) were used to analyze the whole process from the first step of the protocol (sucrose culture) to the recovery of the cryopreserved material. Analyses were carried out through the whole process. Data obtained with RAPDs markers showed always the same mutation in all the analyzed samples, from the high sucrose concentration treatment onwards. On the other hand, differences revealed with the AFLPs markers seemed quite untargeted through the whole-genome level, although the number of differences increased through the process, being the cryopreservd samples those with higher amount of differences within each line. These results suggest that the genetic instability detected with these analyses could be correlated to the degree of stress suffered by the samples during the cryopreservation protocol.
Internacional
Si
Nombre congreso
Working Group 1 meeting of COST Action 871
Tipo de participación
960
Lugar del congreso
Royal Botanic Gardens in Kew, Wakehurst Place, United Kingdom
Revisores
Si
ISBN o ISSN
0143-2044
DOI
Fecha inicio congreso
17/02/2009
Fecha fin congreso
18/02/2009
Desde la página
396
Hasta la página
397
Título de las actas
Cryoletters, Volume: 30 Issue: 5
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Maria Carmen Martin Fernandez (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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