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Descripción
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| In a previous work the characterisation and the alignment of twenty peroxidasegenes together with phylogenetic clustering with peroxidases from other plantspecies showed that these enzymes fall into seven different groups (designatedTaPrx108 to TaPrx114). These represent peroxidases secreted to the apoplast by aputative N-terminal peptide signal. Three groups of peroxidase genes (TaPrx111,TaPrx112 and TaPrx113) were found to be greatly induced by the cereal cystnematode Heterodera avenae in the H-93-8 resistant wheat line. In situ hybridisationanalyses of genes belonging to these groups revealed a strong signal of theseperoxidases in cells close to the vascular cylinder and parenchyma vascular cellswhen challenged by nematodes. In particular, parenchyma cells, where the nematodestarts its feeding, were hyper-reactive to some probes belonging to TaPrx112 andTaPrx113 groups. These findings suggest that wheat apoplastic peroxidases mayhave a role in the defence response. TaPrx111-A, TaPrx112-D and TaPrx113-Fperoxidases were characterised and their sequences were used to design specific pairprimers from each group. Chromosomal analyses by PCR marker mapping andusing Sears¿s aneuploid lines of wheat cv. Chinese Spring indicated that theseperoxidases are located on the short arm of the chromosome 2B and that they may be linked each other. | |
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Internacional
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Si |
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DOI
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Edición del Libro
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0 |
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Editorial del Libro
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CIMMYT |
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ISBN
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978-975-407-285-3 |
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Serie
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Título del Libro
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Cereal Cyst nematodes: Status, research and Outlook |
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Desde página
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195 |
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Hasta página
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199 |