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Abstract
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| Chrysanthemum (Dendranthema grandiflora Tzvelev) is one of the most important crops in the world for cut flowers and pot plants. Due to its economical interest, many new technologies and breeding programmes have been applied to this crop. Although cryopreservation was supposed to be a guarantee for the genetic stability in comparison with other in vitro long term storage procedures, genetic variation in cryopreserved material has been found. Genetic instability of cryopreserved chrysanthemum apices using two different methods has been. In order to evaluate the step of the cryopreservation protocol associated to the variation, we have studied the stability along the encapsulation-dehydration cryopreservation process (pre-culture, encapsulation, dehydration, freeze-treatment and recovery) using RAPDs markers. RAPDs markers were obtained from the amplification of the total genomic DNA isolated from samples (shoots) regenerated after the different steps. Differences in the band pattern were detected not only in cryopreserved samples, but also in the different previous steps. The sequences of the variable bands were analysed through MegAlign (DNAStar) and BLAST/FASTA programmes. | |
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International
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Si |
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Congress
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1st International Symposium on Cryopreservation in Horticultural Species, Arenberg Castle, Heverlee, Belgium |
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960 |
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Place
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Lovaina, Bélgica |
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Reviewers
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Si |
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ISBN/ISSN
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Start Date
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05/04/2010 |
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End Date
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08/05/2010 |
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From page
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0 |
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To page
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0 |
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Acta Horticulturae. En prensa |