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Memorias de investigación
Ponencias en congresos:
DNA sequences of variable bands found after cryopreservation of chrysanthemum apices
Año:2009
Áreas de investigación
  • Producción vegetal
Datos
Descripción
Chrysanthemum (Dendranthema grandiflora Tzvelev) is one of the most important crops in the world for cut flowers and pot plants. Due to its economical interest, many new technologies and breeding programmes have been applied to this crop. Although cryopreservation was supposed to be a guarantee for the genetic stability in comparison with other in vitro long term storage procedures, genetic variation in cryopreserved material has been found. Genetic instability of cryopreserved chrysanthemum apices using two different methods has been. In order to evaluate the step of the cryopreservation protocol associated to the variation, we have studied the stability along the encapsulation-dehydration cryopreservation process (pre-culture, encapsulation, dehydration, freeze-treatment and recovery) using RAPDs markers. RAPDs markers were obtained from the amplification of the total genomic DNA isolated from samples (shoots) regenerated after the different steps. Differences in the band pattern were detected not only in cryopreserved samples, but also in the different previous steps. The sequences of the variable bands were analysed through MegAlign (DNAStar) and BLAST/FASTA programmes.
Internacional
Si
Nombre congreso
1st International Symposium on Cryopreservation in Horticultural Species, Arenberg Castle, Heverlee, Belgium
Tipo de participación
960
Lugar del congreso
Lovaina, Bélgica
Revisores
Si
ISBN o ISSN
DOI
Fecha inicio congreso
05/04/2010
Fecha fin congreso
08/05/2010
Desde la página
0
Hasta la página
0
Título de las actas
Acta Horticulturae. En prensa
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Maria Carmen Martin Fernandez (UPM)
  • Autor: M. Elena Gonzalez Benito (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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