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Descripción
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| Micropropagation isawidelyusedtechniqueinhops(Humulus lupulus L.). However, to thebestofourknowledge,thegeneticandepigeneticstabilityofthemicroplants has neverbeentestedbefore.Inthepresentstudy,twohopaccessionswere established in vitro and micropropagatedfor2years.Thegeneticandepigenetic stability ofthe in vitro plants wasanalyzedwithseveralmoleculartechniques: random amplifiedDNApolymorphism(RAPD),retrotransposonmicrosatellite amplified polymorphism(REMAP),andmethylation-sensitiveamplificationpoly- morphism (MSAP).Nogeneticvariationamongcontrolandtreatedplantswasfound, even after12cyclesofmicropropagation.Epigeneticvariationwasdetected,first, when fieldand in vitro samples werecompared.Nearlya30%ofthedetected fragments presentedthesamepatternofalterationsinallthevitroplants.Second, lower levelsofepigeneticvariationweredetectedamongplantsfromthedifferent subcultures. Partofthisdetectedvariationseemedtobeaccumulatedalongthe12 sequential subculturestested. | |
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Internacional
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JCR del ISI
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Si |
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Título de la revista
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JOURNAL OF PLANT PHYSIOLOGY |
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ISSN
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0176-1617 |
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Factor de impacto JCR
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2,437 |
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Información de impacto
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Volumen
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DOI
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Número de revista
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0 |
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Desde la página
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234 |
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Hasta la página
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243 |
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Mes
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ENERO |
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