Observatorio de I+D+i UPM

Memorias de investigación
Artículos en revistas:
Genomic heterogeneity and host recovery of isolates of Malva vein clearing virus
Año:2009
Áreas de investigación
  • Biología molecular
Datos
Descripción
Malva vein clearing virus (MVCV), a tentative species of the genus Potyvirus, was identified as the causal agent of viral symptoms in Malva sp. weed plants. Amplified viral genomic fragments corresponding to approximately 20% of the 3 terminal region of its genome were obtained using non-species specific, genus-specific reagents. The sequences of the PCR fragments were determined. BLAST and phylogenetic analyses of the deduced amino acid sequence indicated that MVCV is a distinct species of the genus Potyvirus and close to Pea seed-borne mosaic virus (PSbMV) with which it forms a newphylogenetic cluster within the genus. The results show that MVCV is a definitive member of the Potyvirus genus. Specific MVCV PCR primers were designed and validated as diagnostic tools, and used to assess the variability of the species. Much variation was found and this was not correlated with either the geographical origin of the isolates, or the severity of the symptoms. Recovery from viral symptoms was observed in natural and experimental hosts. Tests in experimental hosts showed that it was a true viral recovery, in that the virus was absent and the recovered tissues could not be infected. This is the first reported example of true viral recovery of a potyvirus in a natural system.
Internacional
Si
JCR del ISI
Si
Título de la revista
VIRUS RESEARCH
ISSN
0168-1702
Factor de impacto JCR
2,429
Información de impacto
Volumen
DOI
Número de revista
0
Desde la página
91
Hasta la página
97
Mes
ENERO
Ranking
Esta actividad pertenece a memorias de investigación
Participantes
  • Participante: Angeles Tourino
  • Participante: Pablo Lunello
  • Autor: Flora Sanchez Sanchez (UPM)
  • Participante: Yolanda Nunez
  • Autor: Fernando Ponz Ascaso (UPM)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2021 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)