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Abstract
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| A wide variety of experimental approaches have been developed to study protein-protein interactions in plants, however most of them require disruption of cells or indirect assays of protein detection. The development of sensitive and versatile methods able to detect these interactions in vivo and in homologous tissue and species, is essential to understand protein functions. The Bimolecular Complementation (BiC) either using fluorescent (BiFC) or luminescent (BiLC) compounds provides a direct approach of visualization of molecular interaction in real time, living cells at the subcellular level and under real physiological conditions. BiFC and BiLC procedures are based on the complementation between two fragments of a fluorescent/luminescent protein that can associate when they are brought together by the interaction between proteins fused to each of the fragments. Recent improvements of this technique, the multicolour BiFC approach (mBiFC), have been developed for the simultaneous visualization of multiple protein interactions in the same cell. The combination of fragments of different types of fluorescent proteins makes possible the simultaneous detection of protein complexes in planta. The great potential of this technology to determine biological interactions will be presented. | |
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International
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No |
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Congress
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XIII Congress of the Spanish Society for Cell Biology |
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960 |
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Place
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Murcia |
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Reviewers
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No |
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ISBN/ISSN
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9788474011975 |
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Start Date
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16/12/2009 |
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End Date
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18/12/2009 |
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From page
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48 |
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To page
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48 |
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XIII Congress of SEBC |