Memorias de investigación
Ponencias en congresos:
Towards a digital model of zebrafish embryogenesis. Integration of cell tracking and gene expression quantification
Año:2010
Áreas de investigación
  • Tecnología electrónica y de las comunicaciones
Datos
Descripción
We elaborate on a general framework composed of a set of computational tools to accurately quantificate cellular position and gene expression levels throughout early zebrafish embryogenesis captured over a time-lapse series of in vivo 3D images. Our modeling strategy involves nuclei detection, cell geometries extraction, automatic gene levels quantification and cell tracking to reconstruct cell trajectories and lineage tree which describe the animal development. Each cell in the embryo is then precisely described at each given time t by a vector composed of the cell 3D spatial coordinates (x; y; z) along with its gene expression level g. This comprehensive description of the embryo development is used to assess the general connection between genetic expression and cell movement. We also investigate genetic expression propagation between a cell and its progeny in the lineage tree. More to the point, this paper focuses on the evolution of the expression pattern of transcriptional factor goosecoid (gsc) through the gastrulation process between 6 and 9 hours post fertilization (hpf).
Internacional
Si
Nombre congreso
32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2010)
Tipo de participación
960
Lugar del congreso
Buenos Aires (Argentina)
Revisores
Si
ISBN o ISSN
1557-170X
DOI
10.1109/IEMBS.2010.5626769
Fecha inicio congreso
31/08/2010
Fecha fin congreso
04/09/2010
Desde la página
5520
Hasta la página
5523
Título de las actas
Proceedings of the 32nd Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: Carlos Castro Gonzalez UPM
  • Autor: Louise Douloquin Institut de Neurobiologie Alfred Fessard CNRS
  • Autor: Miguel Angel Luengo Oroz UPM
  • Autor: Sophie Desnoulez Institut de Neurobiologie Alfred Fessard CNRS
  • Autor: Nadine Peyriéras Institut de Neurobiologie Alfred Fessard CNRS
  • Autor: Tierry Savy CREA-École Polytechnique Paris
  • Autor: Camilo Melani CREA-École Polytechnique Paris
  • Autor: Paul Bourgine CREA-École Polytechnique Paris
  • Autor: Maria Jesus Ledesma Carbayo UPM
  • Autor: Andres de Santos Lleo UPM
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Tecnología de imágenes biomédicas
  • Centro o Instituto I+D+i: Centro de tecnología Biomédica CTB
  • Departamento: Ingeniería Electrónica