Memorias de investigación
Research Publications in journals:
A barley cysteine-protease inhibitor reduces teh performance of two aphid species in artificial diets and transgenic arabidopsis plants
Year:2010
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
¿ts have been impli- was to analyze the effects of the whole gene family s, based on their members of barley cystatins against two aphids, cysteine-protein- Myzus persicae and Acyrthosiphon pisum. All the d thirteen genes cystatins, except HvCPI-7, HvCPI-10 and HvCPI-12, to HvCPI-13) from inhibited in vitro the activity of cathepsin L-and/or but only HvCPI-1 B-like proteinases, with HvCPI-6 being the most direct-mutagenesis, effective inhibitor for both aphid species. When The aim of this study administered in artificial diets, HvCPI-6 was toxic to A. pisum nymphs (LC50 = 150 lg/ml), whereas no significant mortality was observed on M. persicae nymphs up to 1000 lg/ml. The effects of HvCPI-6 ingestion on A. pisum were correlated with a decrease of cathepsin B-and L-like proteinase activities. In the (&) Centro de de Maeztu, 9, de Plantas Universidad (km 38), of Crop Ghent Ghent, Belgium case of M. persicae, there was an increase of these proteolytic activities, but also of the aminopeptidase-like activity, suggesting that this species is regulating both target and insensitive enzymes to overcome the effects of the cystatin. To further analyze the potential of barley cystatins as insecticidal proteins against aphids, Arabidopsis plants expressing HvCPI-6 were tested against M. persicae. For A. pisum, which does not feed on Arabidopsis, a combined diet-Vicia faba plant bioassay was performed. A signif-icant delay in the development time to reach the adult stage was observed in both species. The present study demonstrates the potential of barley cystatins to interfere with the performance of two aphid species.
International
Si
JCR
Si
Title
TRANSGENIC RESEARCH
ISBN
0962-8819
Impact factor JCR
2,467
Impact info
Volume
10.1007/s11248-010-9417-2
Journal number
From page
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To page
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Month
ENERO
Ranking
Participants
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.