Memorias de investigación
Research Publications in journals:
Expression of a barley cystatin gene in maize enhances resistance against phytophagous mites by altering their cysteine-proteases
Year:2010
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
The aim of this study was to assess the effects of barley cystatins on two phytophagous spider mites, Tetranychus urticae and Brevipalpus chilensis. The determination of proteolytic activity profile in both mite species showed the presence of the cysteine-proteases, putative targets of cystatins, among other enzymatic activities. All barley cystatins, except HvCPI-1 and HvCPI-7, inhibited in vitro mite cathepsin L- and/or cathepsin B-like activities, HvCPI-6 being the strongest inhibitor for both mite species. Transgenic maize plants expressing HvCPI-6 protein were generated and the functional integrity of the cystatin transgene was confirmed by in vitro inhibitory effect observed against T. urticae and B. chilensis protein extracts. Feeding experiments impaired on transgenic lines performed with T. urticae impaired mite development and reproductive performance. Besides, a significant reduction of cathepsin L-like and/or cathepsin B-like activities was observed when the spider mite fed on maize plants expressing HvCPI-6 cystatin. These findings reveal the potential of barley cystatins as acaricide proteins to protect plants against two important mite pests.
International
Si
JCR
Si
Title
PLANT CELL REPORTS
ISBN
0721-7714
Impact factor JCR
2,301
Impact info
Volume
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10.1007/s00299-010-0948-z
Journal number
From page
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To page
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Month
ENERO
Ranking
Participants
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP