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Ponencias en congresos:
Characterization of Rhodiola extracts by MS and GC-MS
Año:2010
Áreas de investigación
  • Ingenierías,
  • Tecnología química
Datos
Descripción
Rhodiola rosea is a plant belonging to Crassulaceae family, whose root has been used in the popular medicine in Eastern Europe and Asia. It is defined as an adaptogen which produce favourable influence on a diversity of physiological functions (1). These properties are related to the presence of several classes of compounds such as phenyl propanoids, phenylethanol derivatives, polyphenols, terpenes and phenolic acids; this bioactivity and the limited habitat of the plant, make the commercial extracts expensive. The content of bioactive substances in the extracts varies with the geographical origin, culture conditions and extraction process. The quality control of these extracts defines their genuinity and also their content of bioactive substances. Analytical methods have been generally based on HPLC (2, 3) and CE (4). Taking into account the multiplicity of bioactive substances in Rhodiola rosea, we have selected MS and GC-MS as the techniques of choice because their high specificity. Different extracts of the ground roots using water, ethanol and dichloromethane for 3 hours at room temperature were obtained. Extracts were filtered using Whatman nº 40 filter paper. MS analyses were carried out by direct injection at 30 ºC for 0.5 min, then at 70 ºC for 0.5 min and finally at 450 ºC for 10 min. GC-MS analyses of extracts previously converted into their trimethylsilyl oximes (5) were developed using a capillary column coated with methylsilicone as stationary phase. Temperature was held at 60 ºC for 0 min, then raised to 300 ºC at a rate of 5 ºC min-1. The GC-MS method afforded the separation of a high number of compounds, including many previously described as bioactive. Mono- and disaccharides, individual phenyl propanoids and their glycosylated forms were well resolved from other substances. Sedo-heptulose and octaverine were detected by the first time in this plant by GC-MS and direct MS, respectively.
Internacional
Si
Nombre congreso
28th International Symposium on Chromatography ISC 2010
Tipo de participación
960
Lugar del congreso
Valencia
Revisores
Si
ISBN o ISSN
0000000000000
DOI
Fecha inicio congreso
12/09/2010
Fecha fin congreso
16/09/2010
Desde la página
1
Hasta la página
1
Título de las actas
Libro de resúmenes del 28th International Symposium on Chromatography ISC 2010
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: SONIA RODRÍGUEZ-SÁNCHEZ (IQOG-CSIC)
  • Autor: Paloma Diaz Fernandez-Zapata (UPM)
  • Autor: Mª LUZ SANZ MURÍAS (IQOG-CSIC)
  • Autor: JESÚS SANZ PERUCHA (IQOG-CSIC)
  • Autor: ISABEL MARTÍNEZ CASTRO (IQOG-CSIC)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Defensa y Aprovechamiento del Medio Natural
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