Observatorio de I+D+i UPM

Memorias de investigación
Ponencias en congresos:
Fast measurement of cooling and heating rates in standard cryopreservation protocols based on vitrification
Año:2010
Áreas de investigación
  • Biodiversidad,
  • Producción vegetal
Datos
Descripción
Some cryopreservation protocols rely on fast transfer from liquid to glassy state, avoiding the formation of intracellular ice crystals. Ice can be equally formed during cooling and warming processes. Protocols include additives and steps designed to reduce freezing equilibrium (Tf) and increase glass transition (Tg) temperatures, as well as to confer specific protection to cells against several associated stresses generated during the cryopreservation process (cold pre-treatment, dehydration and oxidative stress). These additives and pre-treatments also contribute to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between Tf and Tg. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage.
Internacional
Si
Nombre congreso
47th annual meeting of Society for Cryobiology
Tipo de participación
960
Lugar del congreso
Bristol (Reino Unido)
Revisores
Si
ISBN o ISSN
0011-2240
DOI
Fecha inicio congreso
17/07/2010
Fecha fin congreso
20/07/2010
Desde la página
1
Hasta la página
1
Título de las actas
Fast measurement of cooling and heating rates in standard cryopreservation protocols based on vitrification
Esta actividad pertenece a memorias de investigación
Participantes
  • Autor: M. Elena Gonzalez Benito (UPM)
  • Autor: Antonieo Diego Molina García (Instituto del Frío-ICTAN (CSIC))
  • aUTOR: Aline Schneider Teixeira (Instituto del Frío-ICTAN, CSIC)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Biodiversidad y conservación de recursos fitogenéticos
  • Departamento: Biología Vegetal
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