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Descripción
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| Some cryopreservation protocols rely on fast transfer from liquid to glassy state, avoiding the formation of intracellular ice crystals. Ice can be equally formed during cooling and warming processes. Protocols include additives and steps designed to reduce freezing equilibrium (Tf) and increase glass transition (Tg) temperatures, as well as to confer specific protection to cells against several associated stresses generated during the cryopreservation process (cold pre-treatment, dehydration and oxidative stress). These additives and pre-treatments also contribute to reduce the probability of ice nucleation at all temperatures, mainly through micro-viscosity increase. Still, there is a risk of ice formation in the temperature region comprised between Tf and Tg. Consequently, fast cooling and warming, especially in this region, is a must to avoid ice-derived damage. | |
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Internacional
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Si |
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Nombre congreso
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47th annual meeting of Society for Cryobiology |
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Tipo de participación
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960 |
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Lugar del congreso
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Bristol (Reino Unido) |
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Revisores
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Si |
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ISBN o ISSN
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0011-2240 |
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DOI
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Fecha inicio congreso
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17/07/2010 |
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Fecha fin congreso
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20/07/2010 |
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Desde la página
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1 |
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Hasta la página
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1 |
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Título de las actas
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Fast measurement of cooling and heating rates in standard cryopreservation protocols based on vitrification |