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Memorias de investigación
Ponencias en congresos:
Legume host controls the type of nickel ligands in nodule cytosol, and determines nickel transport and expression of a nickel- dependent enzyme in endosymbiotic bacteria
Áreas de investigación
  • Biología molecular, celular y genética
In natural environments transition metals are often chelated by organic ligands due to their high binding affinity (1). In the case of endosymbiotic bacteria, bacteroids are surrounded by plant cytoplasm in which metal ions are also complexed by different ligands. Some of these ligands are organic acids, which, at the same time, are the main carbon substrate transferred to bacteroids in legume nodules (2). Provision of metals to endosymbiotic bacteria represents a potential limitation for metalloenzyme synthesis inside legume nodules (3, 4). We have observed a strong effect of the legume host on hydrogenase synthesis when the same Rhizobium leguminosarum bv. viciae strain establishes a symbiotic interaction with pea (Pisum sativum) or lentil (Lens sculenta) plants (5). These data, along with the different phenotypes of mutants altered in nickel transport in these two hosts (6), suggest a role for the chemical form of nickel on metal provision to the bacteroid. Using a novel methodology based on normal phase chromatography with a silica column coupled to UV and MS detection systems we have determined that nickel malate and nickel citrate are the main nickel complexes in pea nodules. In contrast, nodules induced by the same strain in lentil plants contain mostly nickel citrate, although low amounts of nickel malate and nickel tartrate are also present. These results are consistent with the concentrations of each free ligand in the nodule cytosol of these hosts as corrected by the affinity constant of each ligand for nickel. The observed differences in the cytosolic profile of organic acids in pea and lentil hosts suggest a different nature of carbon compounds feeding the bacteroid that might affect microsymbiont's metabolism during symbiotic nitrogen fixation.
Nombre congreso
9th European Nitrogen Fixation Conference
Tipo de participación
Lugar del congreso
Ginebra (Suiza)
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Título de las actas
Book of Abstracts 9th European Nitrogen Fixation Conference
Esta actividad pertenece a memorias de investigación
  • Autor: Maria Belen Brito Lopez (UPM)
  • Participante: Carmen del Cacho Vicente (Universidad Complutense de Madrid)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Participante: Maria Concepción Pérez Novillo (Universidad Complutense de Madrid)
  • Participante: Carmen Cámara Rica (Universidad Complutense de Madrid)
Grupos de investigación, Departamentos, Centros e Institutos de I+D+i relacionados
  • Creador: Grupo de Investigación: Asociaciones simbióticas planta-microorganismo
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
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