Observatorio de I+D+i UPM
• ESPAÑOL
• ENGLISH
INVESTIGADORES
ESTRUCTURAS DE I+D+I
ACTIVIDAD INVESTIGADORA
INNOVACIÓN
Investigador
Luis Manuel Rubio Herrero
Categoría Investigador
TITULAR UNIVERSIDAD
Escuela UPM
ETSI AGRONÓMICA, ALIMENT. Y BIOSISTEMAS
Departamento:
BIOTECNOLOGÍA - BIOLOGÍA VEGETAL
Centros/Institutos I+D+i
CENTRO DE BIOTEC. Y GENOMICA DE PLANTAS
Area Conocimiento
MICROBIOLOGIA
Grupo de Investigación:
Abordajes Multidisciplinares en la Interfaz Planta-Microorganismo
Email (Directorio UPM)
lm.rubio
upm.es
Web personal (Directorio UPM)
http://www.cbgp.upm.es/en/nitrogen_fixation.php
Teléfono (Directorio UPM)
910670875
Datos de Memorias relacionados
Proyectos de I+D+i y generación de recursos
22 Proyectos de I+D+i
1: (2011)
AZOTOBACTER VINELANDII COMO BIOFACTORÍA PARA LA PRODUCCIÓN DE HIDRÓGENO
2: (2011)
BIOSISTESIS DEL COFACTOR DE HIERRO Y MOLIBDENO DE LA NITROGENASA
3: (2011)
ENGINEERING NITROGEN FIXATION IN MITOCHONDRIA
4: (2011)
NFIX: Engineering nitrogen fixation in mitochondria
5: (2011)
TOHPN: Towards the optimization of hydrogen production by nitrogenase
6: (2012)
AZOTOBACTER VINELANDII COMO BIOFACTORÍA PARA LA PRODUCCIÓN DE HIDRÓGENO
7: (2012)
BIOSISTESIS DEL COFACTOR DE HIERRO Y MOLIBDENO DE LA NITROGENASA
8: (2012)
ENGINEERING NITROGEN FIXATION IN MITOCHONDRIA
9: (2012)
Ingineering nitrogen fixation in mitochondria
10: (2013)
BIOSISTESIS DEL COFACTOR DE HIERRO Y MOLIBDENO DE LA NITROGENASA
11: (2015)
BIO-N2: Aplicaciones biotecnológicas de la nitrogenasa
12: (2015)
PlantFrontiers: Nuevas fronteras de la biología de plantas
13: (2015)
TOWARDS THE OPTIMIZATION OF HYDROGEN PRODUCTION BY NITROGENASE - TOHPN
14: (2016)
BNF CEREALS: BNF CEREALS PHASE II
15: (2016)
BNF CEREALS PHASE II
16: (2017)
BNF CEREALS PHASE II
17: (2017)
BNF CEREALS PHASE II
18: (2018)
: Sistema de alto rendimiento para la producción fotobiológica de hidrógeno
19: (2018)
FIXIT: Nuevos Paradigmas en Biogenesis de Nitrogenasa
20: (2018)
SUBPROGRAMA ESTATAL DE FORTALECIMIENTO INSTITUCIONAL. CENTROS DE EXCELENCIA SEVERO OCHOA Y UNIDADES DE EXCELENCIA MARÍA DE MAEZTU
21: (2019)
CHARGEREEN
22: (2019)
SymBioM: Laboratorio de Biología de Sistemas y Modelización Biológica del CBGP (UPM-INIA)
Formación de Investigadores y Movilidad
2 Estancias y sabáticos recogidos en programas oficiales
1: (2012)
Estancia en el Dept. of Chemistry of the University of California at Davis
2: (2012)
Estancia en Lehrstuhl für Biologie der Mikroorganismen Ruhr-Universität
6 Tesis Doctorales
1: (2014)
AZOTOBACTER VINELANDII NITROGENASE: KINETICS OF NIF GENE EXPRESSION AND INSIGHTS INTO THE ROLES OF FDXN AND NIFQ IN FEMO-CO BIOSYNTHESIS
2: (2017)
Caracterización de una nueva proteína hipertermófila NifB y su papel en el mecanismo de síntesis de NifB-co, precursor del grupo metálico de FeMo-co.
3: (2017)
Caracterización de una nueva proteína hipertermófila NifB y su papel en el mecanismo de síntesis de NifB-co, precursor del grupo metálico de FeMo-co.
4: (2017)
Characterization of a new hyperthermophilic NifB protein and its role in the mechanism of synthesis of NifB-co, a precursor of the metal cage of FeMo-co
5: (2018)
Synthetic biology tools for the study of relevant factors in the control of transgene
6: (2019)
Molybdenum metabolism in Azotobacter vinelandii and its biotechnological applications
Difusión de Resultados de Investigación
30 Artículos en revistas
1: (2011)
A sterile alpha-motif domain in NafY targets Apo-NifDK for iron-molybdenum cofactor delivery via a tethered domain.
2: (2011)
NifB and NifEN protein levels are regulated by ClpX2 under nitrogen fixation conditions in Azotobacter vinelandii.
3: (2013)
Role of Azotobacter vinelandii FdxN in FeMo-co biosynthesis
4: (2013)
Roles of Four Conserved Basic Amino Acids in a Ferredoxin-Dependent Cyanobacterial Nitrate Reductase
5: (2014)
Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer
6: (2014)
Kinetics of nif Gene Expression in a Nitrogen-Fixing Bacterium
7: (2014)
Role of Azotobacter vinelandii FdxN in FeMo-co biosynthesis
8: (2015)
Cyanobacterial biological nitrogen fixation as a sustainable nitrogen fertilizer for the production of microalgal oil
9: (2016)
Electron Paramagnetic Resonance Characterization of Three Iron-Sulfur Clusters Present in the Nitrogenase Cofactor Maturase NifB from Methanocaldococcus infernus
10: (2016)
Electron Paramagnetic Resonance Characterization of Three Iron?Sulfur Clusters Present in the Nitrogenase Cofactor Maturase NifB from Methanocaldococcus infernus
11: (2016)
EXAFS reveals two Mo environments in the nitrogenase iron-molybdenum cofactor biosynthetic protein NifQ
12: (2016)
Expression of a functional oxygen-labile nitrogenase component in the mitochondrial matrix of aerobically grown yeast
13: (2016)
Hydrogen overproducing nitrogenases obtained by random mutagenesis and high-throughput screening
14: (2016)
The Nitrogenase FeMo-Cofactor Precursor Formed by NifB Protein: A Diamagnetic Cluster Containing Eight Iron Atoms
15: (2017)
Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria
16: (2017)
Challenges to develop nitrogen-fixing cereals by direct nif-gene transfer (vol 225, pg 130, 2014)
17: (2017)
Diversity and Functional Analysis of the FeMo-Cofactor Maturase NifB
18: (2017)
Formation of Nitrogenase NifDK Tetramers in the Mitochondria of Saccharomyces cerevisiae
19: (2017)
Purification and In Vitro Activity of Mitochondria Targeted Nitrogenase Cofactor Maturase NifB
20: (2017)
Purification and In Vitro Activity of Mitochondria Targeted Nitrogenase Cofactor Maturase NifB
21: (2017)
State of the art in eukaryotic nitrogenase engineering
22: (2017)
State of the art in eukaryotic nitrogenase engineering
23: (2017)
State of the art in eukaryotic nitrogenase engineering
24: (2018)
Extreme bioengineering to meet the nitrogen challenge
25: (2018)
Sequential and differential interaction of assembly factors during nitrogenase MoFe protein maturation
26: (2018)
State of the art in eukaryotic nitrogenase engineering
27: (2019)
Biosynthesis of the nitrogenase active-site cofactor precursor NifB-co in Saccharomyces cerevisiae
28: (2019)
Biosynthesis of the nitrogenase active-site cofactor precursor NifB-co in Saccharomyces cerevisiae
29: (2019)
Genetic and Biochemical Analysis of the Azotobacter vinelandii Molybdenum Storage Protein
30: (2019)
Recognition motifs rather than phylogenetic origin influence the ability of targeting peptides to import nuclear-encoded recombinant proteins into rice mitochondria
6 Capítulos de libros
1: (2011)
Biochemistry of Mo-Nitrogenase
2: (2013)
Biosynthesis of the Iron-Molybdenum Cofactor of Nitrogenase
3: (2014)
Expression and Purification of NifB Proteins from Aerobic and Anaerobic Sources
4: (2014)
Purification of O2-Sensitive metalloproteins
5: (2015)
BIOSYNTHESIS OF THE IRON-MOLYBDENUM COFACTOR OF NITROGENASE
6: (2015)
REGULATION OF NIF GENE EXPRESSION IN AZOTOBACTER VINELANDII
24 Conferencias invitadas en congresos
1: (2011)
Changes in nif gene expression profiles during nitrogenase biogenesis.
2: (2011)
The FdxN protein is required for the biosynthesis and activity of the NifDK and NifH nitrogenase component in Azotobacter vinelandii.
3: (2012)
Nitrogenase Biosynthesis: recent advances and perspectives
4: (2012)
Nitrogenase biosynthesis: recent advances and perspectives
5: (2012)
Quantification of Nitrogenase Structural and Biosynthetic Proteins
6: (2013)
Identification of an electron donor to NifB in FeMo-co biosynthesis
7: (2013)
TEDxCibelesChange
8: (2015)
Engineering nitrogenase in eukaryotes
9: (2015)
NITROGENASE ASSEMBLY IN EUKARYOTES
10: (2015)
NITROGENASE BIOTECHNOLOGICAL APPLICATIONS
11: (2016)
Biotechnological applications of nitrogenase
12: (2016)
BNF Cereals Phase II
13: (2016)
Engineering nitrogen fixation
14: (2016)
Expression and Maturation of Nitrogenase Components in Mitochondria
15: (2016)
Expression of Nitrogenase Component Proteins is Eukaryotes. The nitrogen nutrition of plants.
16: (2016)
Nitrogenase assembly in eukaryotes
17: (2016)
Optimizing H2 production by nitrogenase
18: (2016)
Optimizing H2 production by nitrogenase
19: (2016)
Optimizing H2 production by nitrogenase
20: (2016)
Why study nitrogenase? Plant & Microbial Biology
21: (2017)
Advances in engineering eukaryotic nitrogenase
22: (2017)
Advances in engineering eukaryotic nitrogenase
23: (2017)
Innovative Solutions
24: (2019)
Iron Sulfur Discussion Group (2019).
1 Cursos, seminarios y tutoriales
1: (2015)
VII JORNADA CIENTÍFICA CENTRO DE BIOTECNOLOGÍA Y GENÓMICA DE PLANTAS. UPM-INIA (CBGP) 25 de Septiembre de 2015 Sala de Seminarios del CBGP, Campus de Montegancedo UPM
1 Otras Publicaciones
1: (2018)
'Expression of mitochondria targeted NifH in Nicotiana benthamiana'
19 Ponencia en Congresos
1: (2013)
Engineering a hydrogen biosensor in the nitrogen-fixing strain Rhodobacter capsulatus
2: (2013)
Temporal profile of nif gene expression in Azotobacter vinelandii: effect of nifA mutation
3: (2013)
The FdxN protein is required for the biosynthesis and activity of the NifDK nitrogenase components in Azotobacter vinelandii
4: (2013)
Towards the optimization of hydrogen production by nitrogenase: In vitro directed evolution of Rhodobacter capsulatus molybdenum nitrogenase
5: (2013)
Two-Hybrid System in the nitrogen-fixing bacterium Azotobacter vinelandii
6: (2014)
Engineering nitrogen fixation in eukaryots
7: (2014)
Generation of mature NifH variants independent of NifM function
8: (2014)
Nitrogenase and nitrate reductase activities of Azotobacter vinelandii nifO and nifQ mutants growing diazotrophically in the presence of nitrate
9: (2014)
The NifX-like domain found in Klebsiella oxytocaNifB is not required for NifB-co synthesis
10: (2015)
A NOVEL METHOD TO ISOLATE NATIVE NIFB-COFACTOR
11: (2015)
ENGINEERING A HYDROGEN BIOSENSOR: SELECTION OF OVERPRODUCING NITROGENASE VARIANTS FOR BIOHYDROGEN PRODUCTION
12: (2015)
NifQ and NifO are essential to express nitrogenase activity in the presence of nitrate in Azotobacter vinelandii
13: (2016)
Engineering a hydrogen biosensor: selection of overproducing nitrogenase variants for biohydrogen production
14: (2016)
Expression of a functional oxygen-labile nitrogenase component in the mitocondrial matrix of aerobically grown yeast.
15: (2016)
Molybdenum metabolism in Azotobacter vinelandii
16: (2017)
Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of mitochondrial proteins in yeast.
17: (2017)
Diversity and functional analyses of NifB-cofactor maturase (NifB) for its implementation in eukaryotic systems
18: (2017)
Expression of the nitrogenase iron-molybdenum cofactor maturase NifB in mitocondria of Saccharomyces cerevisiae.
19: (2017)
High-throughput screening of hydrogen overproducing photosynthetic bacteria
Explotación de Resultados y Transferencia de Tecnología
4 Patentes
1: (2013)
Reagents and methods for the expression of oxygen-sensitive proteins
2: (2018)
Reagents and Methods for the Expression of an Active NifB Protein and Uses Thereof.
3: (2018)
Reagents and Methods for the Expression of an Active NifB Protein and Uses Thereof.
4: (2018)
Reagents and methods for the expression of oxygen-sensitive proteins
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