Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Identification of a homolog of Arabidopsis DSP4 (SEX4) in chestnut: its induction and accumulation in stem amyloplasts during winter or in response to the cold_
Year:2011
Research Areas
  • Natural sciences and health sciences
Information
Abstract
Oligosaccharide synthesis is an important cryoprotection strategy used by woody plants during winter dormancy. At the onset of autumn, starch stored in the stem and buds is broken down in response to the shorter days and lower temperatures resulting in the buildup of oligosaccharides. Given that the enzyme DSP4 is necessary for diurnal starch degradation in Arabidopsis leaves, this study was designed to address the role of DSP4 in this seasonal process in Castanea sativa Mill. The expression pattern of the CsDSP4 gene in cells of the chestnut stem was found to parallel starch catabolism. In this organ, DSP4 protein levels started to rise at the start of autumn and elevated levels persisted until the onset of spring. In addition, exposure of chestnut plantlets to 4 °C induced the expression of the CsDSP4 gene. In dormant trees or cold-stressed plantlets, the CsDSP4 protein was immunolocalized both in the amyloplast stroma and nucleus of stem cells, whereas in the conditions of vegetative growth, immunofluorescence was only detected in the nucleus. The studies indicate a potential role for DSP4 in starch degradation and cold acclimation following low temperature exposure during activity?dormancy transition
International
Si
JCR
Si
Title
Plant, Cell & Environment
ISBN
0140-7791
Impact factor JCR
5,215
Impact info
Volume
34
10.1111/j.1365-3040.2011.02365.x
Journal number
From page
1693
To page
1704
Month
SIN MES
Ranking
12/190 Plant Sciences
Participants
  • Autor: Maria Carmen Collada Collada (UPM)
  • Autor: Marta Berrocal Lobo (UPM)
  • Autor: Paloma Acebo Pais (UPM)
  • Autor: Alberto Ramos Aranguren (UPM)
  • Autor: Ma. Rosa Casado Garcia (UPM)
  • Autor: Cipriano Aragoncillo Ballesteros (UPM)
  • Autor: Isabel Marta Allona Alberich (UPM)
  • Autor: Cristian Ibáñez (UPM)
  • Autor: Estefanía Pérez Solís (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Biotecnología de Proteínas Vegetales
  • Grupo de Investigación: Genética y Fisiología Forestal
  • Departamento: Biotecnología
S2i 2019 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)