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Memorias de investigación
Communications at congresses:
MOLECULAR CHARACTERIZATION OF A ONE ZINC FINGER TRANSCRIPTION FACTOR FAMILY IN TOMATO
Year:2011
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
Tomato, one of the most important horticultural crops, is quite sensitive to environmental stresses. These stresses cause biochemical, morphological and physiological changes that adversely affect plant growth and productivity. The availability of the draft genome sequence of tomato allows the identification of new genes with potential regulatory roles in controlling the response pathways under such changes. Based on our previous studies of zinc finger transcription factors in Arabidopsis, we identified their potential orthologs in tomato and started complementation assays of the corresponding ko-mutant genes in Arabidopsis. We have performed a phylogenetic study of this family of transcription factors and analysed their expression patterns by RT-qPCR in different plant tissues. In addition, we have determined that there are variations in their expression patterns in response to different abiotic stresses. In vivo DNA-binding and transient expression assays of these transcription factors as fusion proteins with the green fluorescent protein (GFP) allowed us to determine their nuclear localization in support of their regulatory function.
International
Si
Congress
Plant Genomes & Biotechnology meeting.
960
Place
Cold Spring Harbor. USA
Reviewers
No
ISBN/ISSN
Start Date
30/11/2011
End Date
03/12/2011
From page
To page
Participants
  • Autor: Jesus Vicente Carbajosa (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Redes Reguladoras del Desarrollo y Respuesta a Estrés en Plantas
  • Departamento: Biotecnología
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