Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Gene pyramiding of peptidase inhibitors enhances plant resistance to the spider Mmte Tetranychus urticae
Year:2012
Research Areas
  • Natural sciences and health sciences,
  • Molecular biology
Information
Abstract
The two-spotted spider mite Tetranychus urticae is a damaging pest worldwide with a wide range of host plants and an extreme record of pesticide resistance. Recently, the complete T. urticae genome has been published and showed a proliferation of gene families associated with digestion and detoxification of plant secondary compounds which supports its polyphagous behaviour. To overcome spider mite adaptability a gene pyramiding approach has been developed by coexpressing two barley proteases inhibitors, the cystatin Icy6 and the trypsin inhibitor Itr1 genes in Arabidopsis plants by Agrobacterium-mediated transformation. The presence and expression of both transgenes was studied by conventional and quantitative real time RT-PCR assays and by indirect ELISA assays. The inhibitory activity of cystatin and trypsin inhibitor was in vitro analysed using specific substrates. Single and double transformants were used to assess the effects of spider mite infestation. Double transformed lines showed the lowest damaged leaf area in comparison to single transformants and nontransformed controls and different accumulation of H2O2 as defence response in the leaf feeding site, detected by diaminobenzidine staining. Additionally, an impact on endogenous mite cathepsin B- and L-like activities was observed after feeding on Arabidopsis lines, which correlates with a significant increase in the mortality of mites fed on transformed plants. These effects were analysed in view of the expression levels of the target mite protease genes, C1A cysteine peptidase and S1 serine peptidase, identified in the four developmental mite stages (embryo, larvae, nymphs and adults) performed using the RNA-seq information available at the BOGAS T. urticae database. The potential of pyramiding different classes of plant protease inhibitors to prevent plant damage caused by mites as a new tool to prevent pest resistance and to improve pest control is discussed
International
Si
JCR
Si
Title
Plos One
ISBN
1932-6203
Impact factor JCR
4,411
Impact info
Datos JCR del año 2010
Volume
7
10.1371/journal.pone.0043011
Journal number
7
From page
43011
To page
43020
Month
SIN MES
Ranking
Participants
  • Autor: Estrella Santamaria (Universidad de Ontario-Canada)
  • Autor: Ines Cambra Marin (UPM)
  • Autor: Manuel Martinez Muñoz (UPM)
  • Autor: Clara Pozancos (CBGP)
  • Autor: Pablo Gonzalez-Melendi De Leon (UPM)
  • Autor: Vojislava Grbic (Universidad de Ontario-Canada)
  • Autor: Pedro Castañera (CIB-CSIC)
  • Autor: Felix Ortego (CIB-CSIC)
  • Autor: M. Isabel Diaz Rodriguez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Interacciones moleculares planta-insecto.
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
  • Departamento: Biotecnología
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