Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
The Role of Evolutionary Intermediates in the Host Adaptation of Canine Parvovirus
Year:2012
Research Areas
  • Virology,
  • Biodiversity
Information
Abstract
The adaptation of viruses to new hosts is a poorly understood process likely involving a variety of viral structures and functions that allow efficient replication and spread. Canine parvovirus (CPV) emerged in the late 1970s as a host-range variant of a virus related to feline panleukopenia virus (FPV). Within a few years of its emergence in dogs, there was a worldwide replacement of the initial virus strain (CPV type 2) by a variant (CPV type 2a) characterized by four amino acid differences in the capsid protein. However, the evolutionary processes that underlie the acquisition of these four mutations, as well as their effects on viral fitness, both singly and in combination, are still uncertain. Using a comprehensive experimental analysis of multiple intermediate mutational combinations, we show that these four capsid mutations act in concert to alter antigenicity, cell receptor binding, and relative in vitro growth in feline cells. Hence, host adaptation involved complex interactions among both surface-exposed and buried capsid mutations that together altered cell infection and immune escape properties of the viruses. Notably, most intermediate viral genotypes containing different combinations of the four key amino acids possessed markedly lower fitness than the wild-type viruses.
International
Si
JCR
Si
Title
Journal of Virology
ISBN
0022-538X
Impact factor JCR
5,402
Impact info
Volume
86
10.1128/JVI.06222-11
Journal number
From page
1514
To page
1521
Month
SIN MES
Ranking
Participants
  • Autor: K. M. Stucker
  • Autor: Jesus Israel Pagan Muñoz (UPM)
  • Autor: J. O. Cifuente
  • Autor: J. T. Kaelber
  • Autor: T. D. Lillie
  • Autor: S. Hafenstein
  • Autor: E. C. Holmes
  • Autor: C. R. Parrish
Research Group, Departaments and Institutes related
  • Creador: Grupo de Investigación: Patología Vegetal
  • Centro o Instituto I+D+i: Centro de Biotecnología y Genómica de Plantas, CBGP
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)