Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Characterization and extraction of the synaptic apposition surface for synaptic geometry analysis
Year:2013
Research Areas
  • Structural biology
Information
Abstract
Geometrical features of chemical synapses are relevant to their function. Two critical components of the synaptic junction are the active zone (AZ) and the postsynaptic density (PSD), as they are related to the probability of synaptic release and the number of postsynaptic receptors, respectively. Morphological studies of these structures are greatly facilitated by the use of recent electron microscopy techniques, such as combined focused ion beam milling and scanning electron microscopy (FIB/SEM), and software tools that permit reconstruction of large numbers of synapses in three dimensions. Since the AZ and the PSD are in close apposition and have a similar surface area, they can be represented by a single surface?the synaptic apposition surface (SAS). We have developed an efficient computational technique to automatically extract this surface from synaptic junctions that have previously been three-dimensionally reconstructed from actual tissue samples imaged by automated FIB/SEM. Given its relationship with the release probability and the number of postsynaptic receptors, the surface area of the SAS is a functionally relevant measure of the size of a synapse that can complement other geometrical features like the volume of the reconstructed synaptic junction, the equivalent ellipsoid size and the Feret's diameter.
International
Si
JCR
Si
Title
Frontiers in Neuroanatomy
ISBN
1662-5129
Impact factor JCR
Impact info
Volume
7
10.3389/fnana.2013.00020
Journal number
20
From page
1
To page
10
Month
JULIO
Ranking
Participants
  • Autor: Juan Morales Del Olmo (UPM)
  • Autor: Angel Rodriguez Mtnez.de Bartolome (UPM)
  • Autor: José Rodrigo Rodríguez
  • Autor: javier defelipe
  • Autor: Angel Merchan Perez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Arquitectura y Tecnología de Sistemas Informáticos
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)