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Memorias de investigación
Communications at congresses:
DmeRF system is requiered for nickel and cobalt resistance in Rhizobium leguminosarum bv. viciae
Year:2013
Research Areas
  • Molecular, cellular and genetic biology
Information
Abstract
A gene encoding a homolog to the cation diffusion facilitator protein DmeF from Cupriavidus metallidurans has been identified in the genome of Rhizobium leguminosarum UPM791. The R. leguminosarum dmeF gene is located downstream of an open reading frame (designated dmeR) encoding a protein homologous to the nickel- and cobalt-responsive transcriptional regulator RcnR from Escherichia coli. Analysis of gene expression showed that the R. leguminosarum dmeRF genes are organized as a transcriptional unit whose expression is strongly induced by nickel and cobalt ions, likely by alleviating the repressor activity of DmeR on dmeRF transcription. An R. leguminosarum dmeRF mutant strain displayed increased sensitivity to Co(II) and Ni(II), whereas no alterations of its resistance to Cd(II), Cu(II), or Zn(II) were observed. A decrease of symbiotic performance was observed when pea plants inoculated with an R. leguminosarum dmeRF deletion mutant strain were grown in the presence of high concentrations of nickel and cobalt. The same mutant induced significantly lower activity levels of NiFe hydrogenase in microaerobic cultures. These results indicate that the R. leguminosarum DmeRF system is a metal-responsive efflux mechanism acting as a key element for metal homeostasis in R. leguminosarum under free-living and symbiotic conditions. The presence of similar dmeRF gene clusters in other Rhizobiaceae suggests that the dmeRF system is a conserved mechanism for metal tolerance in legume endosymbiotic bacteria.
International
No
Congress
II IBEMPA, Congreso de la Sociedad Española de Fijación de Nitrógeno (SEFIN) y la Asociación Latinoamericana de Rhizobiología (ALAR)
960
Place
Sevilla, España
Reviewers
Si
ISBN/ISSN
Start Date
02/09/2013
End Date
06/09/2013
From page
To page
Participants
  • Autor: Laura Rubio Sanz (UPM)
  • Autor: Rosa Isabel Prieto Carbajo (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Maria Belen Brito Lopez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Química y Análisis Agrícola
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