Observatorio de I+D+i UPM

Memorias de investigación
Communications at congresses:
DmeRF system is requiered for nickel and cobalt resistance in Rhizobium leguminosarum bv. viciae
Research Areas
  • Molecular, cellular and genetic biology
A gene encoding a homolog to the cation diffusion facilitator protein DmeF from Cupriavidus metallidurans has been identified in the genome of Rhizobium leguminosarum UPM791. The R. leguminosarum dmeF gene is located downstream of an open reading frame (designated dmeR) encoding a protein homologous to the nickel- and cobalt-responsive transcriptional regulator RcnR from Escherichia coli. Analysis of gene expression showed that the R. leguminosarum dmeRF genes are organized as a transcriptional unit whose expression is strongly induced by nickel and cobalt ions, likely by alleviating the repressor activity of DmeR on dmeRF transcription. An R. leguminosarum dmeRF mutant strain displayed increased sensitivity to Co(II) and Ni(II), whereas no alterations of its resistance to Cd(II), Cu(II), or Zn(II) were observed. A decrease of symbiotic performance was observed when pea plants inoculated with an R. leguminosarum dmeRF deletion mutant strain were grown in the presence of high concentrations of nickel and cobalt. The same mutant induced significantly lower activity levels of NiFe hydrogenase in microaerobic cultures. These results indicate that the R. leguminosarum DmeRF system is a metal-responsive efflux mechanism acting as a key element for metal homeostasis in R. leguminosarum under free-living and symbiotic conditions. The presence of similar dmeRF gene clusters in other Rhizobiaceae suggests that the dmeRF system is a conserved mechanism for metal tolerance in legume endosymbiotic bacteria.
II IBEMPA, Congreso de la Sociedad Española de Fijación de Nitrógeno (SEFIN) y la Asociación Latinoamericana de Rhizobiología (ALAR)
Sevilla, España
Start Date
End Date
From page
To page
  • Autor: Laura Rubio Sanz (UPM)
  • Autor: Rosa Isabel Prieto Carbajo (UPM)
  • Autor: Jose Manuel Palacios Alberti (UPM)
  • Autor: Maria Belen Brito Lopez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Química y Análisis Agrícola
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)