Observatorio de I+D+i UPM

Memorias de investigación
Research Publications in journals:
Plantation forestry under global warming: hybrid poplars with improved thermotolerance provide new insights on the in vivo function of small-HSP chaperones
Year:2014
Research Areas
  • Molecular biology
Information
Abstract
Climate-driven heat stress is a key factor affecting forest plantation yields. While its effects are expected to worsen during this century, breeding more tolerant genotypes has proven elusive. We report here a substantial and durable increase in the thermotolerance of hybrid poplar (Populus tremula 3 Populus alba) through overexpression of a major small heat shock protein (sHSP) with convenient features. Experimental evidence was obtained linking protective effects in the transgenic events with the unique chaperone activity of sHSPs. In addition, significant positive correlations were observed between phenotype strength and heterologous sHSP accumulation. The remarkable baseline levels of transgene product (up to 1.8% of total leaf protein) have not been reported in analogous studies with herbaceous species. As judged by protein analyses, such an accumulation is not matched either by endogenous sHSPs in both heat-stressed poplar plants and field-grown adult trees. Quantitative real timepolymerase chain reaction analyses supported these observations and allowed us to identify the poplar members most responsive to heat stress. Interestingly, sHSP overaccumulation was not associated with pleiotropic effects that might decrease yields. The poplar lines developed here also outperformed controls under in vitro and ex vitro culture conditions (callus biomass, shoot production, and ex vitro survival), even in the absence of thermal stress. These results reinforce the feasibility of improving valuable genotypes for plantation forestry, a field where in vitro recalcitrance, long breeding cycles, and other practical factors constrain conventional genetic approaches. They also provide new insights into the biological functions of the least understood family of heat shock protein chaperones.
International
Si
JCR
Si
Title
Plant Physiology
ISBN
0032-0889
Impact factor JCR
6,535
Impact info
Datos JCR del año 2011
Volume
10.1104/pp.113.225730
Journal number
164
From page
1
To page
14
Month
FEBRERO
Ranking
Participants
  • Autor: Irene María Merino Sierra (UPM)
  • Autor: Ángela Bibiana Contreras Mogollon (UPM)
  • Autor: Zhong-Ping Jing (Universidad de Málaga)
  • Autor: Fernando Gallardo (Universidad de Málaga)
  • Autor: Francisco Cánovas (Universidad de Málaga)
  • Autor: Luis Gomez Fernandez (UPM)
Research Group, Departaments and Institutes related
  • Creador: Departamento: Sistemas y Recursos Naturales
S2i 2020 Observatorio de investigación @ UPM con la colaboración del Consejo Social UPM
Cofinanciación del MINECO en el marco del Programa INNCIDE 2011 (OTR-2011-0236)
Cofinanciación del MINECO en el marco del Programa INNPACTO (IPT-020000-2010-22)